Recombinant human MAP4K1 protein (ab107953)

Overview

Description

  • NatureRecombinant
  • SourceBaculovirus infected Sf9 cells
  • Amino Acid Sequence
    • AccessionQ92918
    • SpeciesHuman
    • Molecular weight65 kDa including tags
    • Amino acids1 to 346

Specifications

Our Abpromise guarantee covers the use of ab107953 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityThe Specific activity of ab107953 was determined to be 125 nmol/min/mg.
  • Applications

    SDS-PAGE

    Western blot

    Functional Studies

  • Purity> 75 % SDS-PAGE.
    Purity was determined to be >75% by densitometry.
  • FormLiquid
  • Additional notes

    ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • Hematopoietic progenitor kinase
    • Hematopoietic progenitor kinase 1
    • HPK1
    • Human Hematopoietic Progenitor Kinase
    • M4K1_HUMAN
    • Map4k1
    • MAPK/ERK kinase kinase kinase 1
    • MEK kinase kinase 1
    • MEKKK 1
    • Mitogen activated protein kinase kinase kinase kinase 1
    • Mitogen-activated protein kinase kinase kinase kinase 1
    see all
  • FunctionMay play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway. May play a role in hematopoietic lineage decisions and growth regulation.
  • Tissue specificityExpressed primarily in hematopoietic organs, including bone marrow, spleen and thymus. Also expressed at very low levels in lung, kidney, mammary glands and small intestine.
  • Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 CNH domain.
    Contains 1 protein kinase domain.
  • Information by UniProt

Recombinant human MAP4K1 protein images

  • SDS-PAGE showing ab107953 at approximately 65kDa.
  • Kinase Assay demonstrating specific activity of ab107953.

References for Recombinant human MAP4K1 protein (ab107953)

ab107953 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. The tag for this protein is a GST tag. Please let me know if I can be of further assistance.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"