Recombinant human MAP4K1/HPK1 protein (ab107953)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 75% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, WB, Functional Studies
Description
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Product name
Recombinant human MAP4K1/HPK1 protein
See all MAP4K1/HPK1 proteins and peptides -
Biological activity
The Specific activity of ab107953 was determined to be 125 nmol/min/mg.
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Purity
> 75 % SDS-PAGE.
Purity was determined to be >75% by densitometry. Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
65 kDa including tags -
Amino acids
1 to 346
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Associated products
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Related Products
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Substrate reagent
Specifications
Our Abpromise guarantee covers the use of ab107953 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Western blot
Functional Studies
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Form
Liquid -
Additional notes
ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
This product was previously labelled as MAP4K1
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.307% Glutathione, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- Hematopoietic progenitor kinase
- Hematopoietic progenitor kinase 1
- HPK1
see all -
Function
May play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway. May play a role in hematopoietic lineage decisions and growth regulation. -
Tissue specificity
Expressed primarily in hematopoietic organs, including bone marrow, spleen and thymus. Also expressed at very low levels in lung, kidney, mammary glands and small intestine. -
Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CNH domain.
Contains 1 protein kinase domain. - Information by UniProt
Images
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The specific activity of MAP4K1/HPK1 (ab107953) was determined to be 110 nmol/min/mg as per activity assay protocol and was equivalent to 144 nmol/min/mg as per radiometric assay
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SDS PAGE analysis of ab107953
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SDS PAGE analysis of ab107953
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SDS-PAGE showing ab107953 at approximately 65kDa.
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Kinase Assay demonstrating specific activity of ab107953.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab107953 has not yet been referenced specifically in any publications.