Recombinant human MAP4K4/NIK protein (ab101775)
Key features and details
- Expression system: Baculovirus infected insect cells
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: WB, SDS-PAGE, Functional Studies
Description
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Product name
Recombinant human MAP4K4/NIK protein -
Biological activity
The specific activity of ab101775 was determined to be 54 nmol/min/mg.
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Purity
> 95 % SDS-PAGE.
Purity was determined to be >95% by densitometry. Affinity purified. -
Expression system
Baculovirus infected insect cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
64 kDa including tags -
Amino acids
1 to 328
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Associated products
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Substrate reagent
Specifications
Our Abpromise guarantee covers the use of ab101775 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
SDS-PAGE
Functional Studies
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Form
Liquid -
Additional notes
ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
This product was previously labelled as MAP4K4
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.307% Glutathione, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- FLH21957
- FLJ10410
- FLJ20373
see all -
Function
Serine/threonine kinase that may play a role in the response to environmental stress and cytokines such as TNF-alpha. Appears to act upstream of the JUN N-terminal pathway. -
Tissue specificity
Appears to be ubiquitous. Expressed in all tissue types examined. Isoform 5 appears to be more abundant in the brain. Isoform 4 is predominant in the liver, skeletal muscle and placenta. -
Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CNH domain.
Contains 1 protein kinase domain. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cytoplasm. - Information by UniProt
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab101775 has not yet been referenced specifically in any publications.