Recombinant human MAP4K4 protein (ab101775)

Overview

Description

  • NatureRecombinant
  • SourceBaculovirus
  • Amino Acid Sequence
    • AccessionO95819
    • SpeciesHuman
    • Molecular weight64 kDa including tags
    • Amino acids1 to 328

Associated products

Specifications

Our Abpromise guarantee covers the use of ab101775 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityThe specific activity of ab101775 was determined to be 54 nmol/min/mg.
  • Applications

    Western blot

    SDS-PAGE

    Functional Studies

  • Purity> 95 % SDS-PAGE.
    Purity was determined to be >95% by densitometry.
  • FormLiquid
  • Additional notes

    ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • FLH21957
    • FLJ10410
    • FLJ20373
    • FLJ90111
    • Hepatocyte progenitor kinase like/germinal center kinase like kinase
    • HGK
    • HPK/GCK like kinase
    • HPK/GCK like kinase HGK
    • HPK/GCK-like kinase HGK
    • KIAA0687
    • M4K4_HUMAN
    • MAP4K4
    • MAPK/ERK kinase kinase kinase 4
    • MEK kinase kinase 4
    • MEKKK 4
    • MEKKK4
    • Mitogen activated protein kinase kinase kinase kinase 4
    • Mitogen-activated protein kinase kinase kinase kinase 4
    • Nck interacting kinase
    • Nck-interacting kinase
    • NIK
    see all
  • FunctionSerine/threonine kinase that may play a role in the response to environmental stress and cytokines such as TNF-alpha. Appears to act upstream of the JUN N-terminal pathway.
  • Tissue specificityAppears to be ubiquitous. Expressed in all tissue types examined. Isoform 5 appears to be more abundant in the brain. Isoform 4 is predominant in the liver, skeletal muscle and placenta.
  • Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 CNH domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationCytoplasm.
  • Information by UniProt

Recombinant human MAP4K4 protein images

  • SDS-PAGE analysis of ab101775.
  • The specific activity of ab101775 was determined to be 54 nmol/min/mg.

References for Recombinant human MAP4K4 protein (ab101775)

ab101775 has not yet been referenced specifically in any publications.

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