Recombinant human MAPKAP Kinase 2 protein (ab60307)

Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Molecular weight
      68 kDa
    • Amino acids
      46 to 400
    • Tags
      GST tag N-Terminus
    • Additional sequence information
      Recombinant fragment, corresponding to amino acids 46-end of Human MAPKAP Kinase 2.

Associated products

Specifications

Our Abpromise guarantee covers the use of ab60307 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Specific activity: 254 nmol/min/mg.
  • Applications

    Functional Studies

    SDS-PAGE

  • Form
    Liquid
  • Additional notes

    ab204861 (Hsp27 peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • MAP kinase activated protein Kinase 2
    • MAP kinase-activated protein kinase 2
    • MAPK activated protein kinase 2
    • MAPK-activated protein kinase 2
    • MAPK2_HUMAN
    • MAPKAP K2
    • MAPKAP kinase 2
    • MAPKAPK 2
    • MAPKAPK-2
    • MAPKAPK2
    • Mitogen activated protein kinase activated protein kinase 2
    • MK 2
    • MK2
    see all
  • Function
    Its physiological substrate seems to be the small heat shock protein (HSP27/HSP25). In vitro can phosphorylate glycogen synthase at 'Ser-7' and tyrosine hydroxylase (on 'Ser-19' and 'Ser-40'). This kinase phosphorylates Ser in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue (By similarity). Mediates both ERK and p38 MAPK/MAPK14 dependent neutrophil responses. Participates in TNF alpha-stimulated exocytosis of secretory vesicles in neutrophils. Plays a role in phagocytosis-induced respiratory burst activity.
  • Tissue specificity
    Expressed in all tissues examined.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylated and activated by MAP kinase.
  • Information by UniProt

Images

  • Sample Kinase Activity Plot.
  • ab60307 on SDS-PAGE.

References

ab60307 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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