The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Protein concentration is above or equal to 0.05 mg/ml.
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Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00 Constituents: 0.31% Glutathione, 0.79% Tris HCl
E3 ubiquitin-protein ligase MARCH5
Membrane-associated ring finger (C3HC4) 5
Membrane-associated RING finger protein 5
Membrane-associated RING-CH protein V
Mitochondrial ubiquitin ligase
RING finger protein 153
Mitochondrial E3 ubiquitin-protein ligase that plays a crucial role in the control of mitochondrial morphology by acting as a positive regulator of mitochondrial fission. May play a role in the prevention of cell senescence acting as a regulator of mitochondrial quality control. Promotes ubiquitination of FIS1, DNM1L and MFN1.
Expressed in brain, heart, liver, lung, spleen, stomach, testis, skeletal and muscle.
Protein modification; protein ubiquitination.
Contains 1 RING-CH-type zinc finger.
The RING-CH-type zinc finger domain is required for E3 ligase activity.
Autoubiquitinated leading to degradation (short half-life).
Mitochondrion outer membrane. Endoplasmic reticulum membrane. Authors show that the protein can be detected in endoplasmic reticulum (PubMed:14722266). Authors (PubMed:16874301) show its presence only in mitochondria.