Overview

Description

  • NatureRecombinant
  • SourceBaculovirus infected Sf9 cells
  • Amino Acid Sequence
    • AccessionQ7KZI7
    • SpeciesHuman
    • Molecular weight114 kDa including tags
    • Amino acids1 to 788

Specifications

Our Abpromise guarantee covers the use of ab119135 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activitySpecific Activity: 725 - 795 nmol/min/mg
  • Applications

    SDS-PAGE

    Western blot

    Functional Studies

  • Purity> 70 % SDS-PAGE.
    Purity was determined to be >70% by densitometry.
  • FormLiquid
  • Additional notes

    ab204854 (Cdc25C peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.88% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • ELKL motif kinase
    • ELKL motif kinase 1
    • EMK-1
    • EMK1
    • MAP/microtubule affinity regulating kinase 2
    • MAP/microtubule affinity-regulating kinase 2
    • Mark2
    • MARK2_HUMAN
    • MGC99619
    • PAR 1
    • Par 1b
    • PAR1 homolog
    • Par1b
    • Ser/Thr protein kinase PAR 1B
    • Serine/threonine protein kinase EMK
    • Serine/threonine protein kinase MARK2
    • Serine/threonine-protein kinase MARK2
    see all
  • FunctionRole in epithelial morphogenesis. Modulates the developmental decision to build a columnar versus a hepatic epithelial cell apparently by promoting a switch from a direct to a transcytotic mode of apical protein delivery. Essential for the asymmetric development of membrane domains of polarized epithelial cells. One or more isoforms may play a role in graft rejection.
  • Tissue specificityHigh levels of expression in heart, brain, skeletal muscle and pancreas, lower levels observed in lung, liver and kidney.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. MARK subfamily.
    Contains 1 KA1 (kinase-associated) domain.
    Contains 1 protein kinase domain.
    Contains 1 UBA domain.
  • Cellular localizationCell membrane. Phosphorylated by PRKCZ in polarized epithelial cells, resulting in an interaction with YWHAZ which promotes relocation from the lateral to the apical membrane.
  • Information by UniProt

Recombinant human MARK2 protein images

  • Sample Kinase Activity Plot: The specific activity of ab119135 was determined to be 795 nmol/min/mg.
  • SDS-PAGE analysis of ab119135.

References for Recombinant human MARK2 protein (ab119135)

ab119135 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"