Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      MASMTGGQQMGRGHHHHHHENLYFQGGEFERKSPSGKKFRSKPQLARYLG GSMDLSTFDFRTGKMLMSKMNKSRQRVRYDSSNQVKGKPDLNTALPVRQT ASIFKQPVTKITNHPSNKVKSDPQKAVDQPRQLFWEKKLSGLNAFDIAEE LVKTMDLPKGLQGVGPGCTDETLLSAIASALHTSTMPITGQLSAAVEKNP GVWLNTTQPLCKAFMVTDEDIRKQEELVQQVRKRLEEALMADMLAHVEEL ARDGEAPLDKACAEDDDEEDEEEEEEEPDPDPEMEHV
    • Molecular weight
      32 kDa including tags
    • Amino acids
      2 to 259
    • Additional sequence information
      Constructed with a small T7-His-TEV cleavage site Tag (31 amino acids) fusion at the N-terminal.

Specifications

Our Abpromise guarantee covers the use of ab185594 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Purity
    >90% by SDS-PAGE.
    ab185594 was expressed in E. coli as inclusion bodies, refolded using a unique temperature shift inclusion body refolding technology and chromatographically purified.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituent: 0.32% Tris HCl

    Contains NaCl, EDTA, KCl, EDTA, arginine, DTT and Glycerol.

General Info

  • Alternative names
    • AI181826
    • AU019209
    • MBD 3
    • Mbd3
    • MBD3: methyl CpG binding domain protein 3
    • MBD3_HUMAN
    • Methyl CpG binding domain protein 3
    • Methyl CpG binding protein MBD3
    • Methyl-CpG-binding domain protein 3
    • Methyl-CpG-binding protein MBD3
    see all
  • Function
    Does not bind DNA by itself. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing.
  • Sequence similarities
    Contains 1 MBD (methyl-CpG-binding) domain.
  • Cellular localization
    Nucleus. Nuclear, in discrete foci.
  • Information by UniProt

References

ab185594 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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