Overview

Description

  • Nature
    Recombinant
  • Source
    Wheat germ
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      MDLAAAAEPGAGSQHLEVRDEVAEKCQKLFLDFLEEFQSSDGEIKYLQLA EELIRPERNTLVVSFVDLEQFNQQLSTTIQEEFYRVYPYLCRALKTFVKD RKEIPLAKDFYVAFQDLPTRHKIRELTSSRIGLLTRISGQVVRTHPVHPE LVSGTFLCLDCQTVIRDVEQQFKYTQPNICRNPVCANRRRFLLDTNKSRF VDFQKVRIQETQAELPRGSIPRSLEVILRAEAVESAQAGDKCDFTGTLIV VPDVSKLSTPGARAETNSRVSGVDGYETEGIRGLRALGVRDLSYRLVFLA CCVAPTNPRFGGKELRDEEQTAESIKNQMTVKEWEKVFEMSQDKNLYHNL CTSLFPTIHGNDEVKRGVLLMLFGGVPKTTGEGTSLRGDINVCIVGDPST AKSQFLKHVEEFSPRAVYTSGKASSAAGLTAAVVRDEESHEFVIEAGALM LADNGVCCIDEFDKMDVRDQVAIHEAMEQQTISITKAGVKATLNARTSIL AAANPISGHYDRSKSLKQNINLSAPIMSRFDLFFILVDECNEVTDYAIAR RIVDLHSRIEESIDRVYSLDDIRRYLLFARQFKPKISKESEDFIVEQYKH LRQRDGSGVTKSSWRITVRQLESMIRLSEAMARMHCCDEVQPKHVKEAFR LLNKSIIRVETPDVNLDQEEEIQMEVDEGAGGINGHADSPAPVNGINGYN EDINQESAPKASLRLGFSEYCRISNLIVLHLRKVEEEEDESALKRSELVN WYLKEIESEIDSEEELINKKRIIEKVIHRLTHYDHVLIELTQAGLKGSTE GSESYEEDPYLVVNPNYLLED
    • Molecular weight
      116 kDa including tags
    • Amino acids
      1 to 821

Specifications

Our Abpromise guarantee covers the use of ab114758 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    ELISA

    SDS-PAGE

    Western blot

  • Form
    Liquid
  • Additional notes
    Protein concentration is above or equal to 0.05 µg/ul.
    Best use within three months from the date of receipt of this protein.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.3% Glutathione, 0.79% Tris HCl

General Info

  • Alternative names
    • DNA replication licensing factor MCM6
    • MCG40308
    • Mcm6
    • MCM6 minichromosome maintenance deficient 6
    • MCM6 minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe)
    • MCM6_HUMAN
    • Minichromosome maintenance complex component 6
    • Minichromosome maintenance deficient (mis5 S. pombe) 6
    • Minichromosome maintenance deficient 6 homolog
    • Minichromosome maintenance protein 6
    • Mis5
    • MIS5 homolog
    • p105MCM
    see all
  • Function
    Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.
  • Sequence similarities
    Belongs to the MCM family.
    Contains 1 MCM domain.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus. Binds to chromatin during G1 and detach from it during S phase.
  • Information by UniProt

Recombinant Human MCM6 protein images

  • 12.5% SDS-PAGE Stained with Coomassie Blue showing ab114758 at approximately 116.38kDa.

References for Recombinant Human MCM6 protein (ab114758)

ab114758 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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