The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Additional notesProtein concentration is above or equal to 0.05 µg/ul. Best use within three months from the date of receipt of this protein.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00 Constituents: 0.3% Glutathione, 0.79% Tris HCl
DNA replication licensing factor MCM6
MCM6 minichromosome maintenance deficient 6
MCM6 minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe)
Minichromosome maintenance complex component 6
Minichromosome maintenance deficient (mis5 S. pombe) 6
Minichromosome maintenance deficient 6 homolog
Minichromosome maintenance protein 6
FunctionActs as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.
Sequence similaritiesBelongs to the MCM family. Contains 1 MCM domain.
Post-translational modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus. Binds to chromatin during G1 and detach from it during S phase.