Overview

Description

  • Nature
    Recombinant
  • Source
    Wheat germ
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      MALKDYALEKEKVKKFLQEFYQDDELGKKQFKYGNQLVRLAHREQVALYV DLDDVAEDDPELVDSICENARRYAKLFADAVQELLPQYKEREVVNKDVLD VYIEHRLMMEQRSRDPGMVRSPQNQYPAELMRRFELYFQGPSSSKPRVIR EVRADSVGKLVTVRGIVTRVSEVKPKMVVATYTCDQCGAETYQPIQSPTF MPLIMCPSQECQTNRSGGRLYLQTRGSRFIKFQEMKMQEHSDQVPVGNIP RSITVLVEGENTRIAQPGDHVSVTGIFLPILRTGFRQVVQGLLSETYLEA HRIVKMNKSEDDESGAGELTREELRQIADVIFATVRELVSGGRSVRFSEA EQRCVSRGFTPAQFQAALDEYEELNVWQVNASRTRITFV
    • Molecular weight
      69 kDa including tags
    • Amino acids
      1 to 389

Specifications

Our Abpromise guarantee covers the use of ab114356 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    ELISA

    Western blot

    SDS-PAGE

  • Form
    Liquid
  • Additional notes
    Protein concentration is above or equal to 0.05 mg/ml.
    Best use within three months from the date of receipt of this protein.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.3% Glutathione, 0.79% Tris HCl

General Info

  • Alternative names
    • CDABP0042
    • CDC 47
    • CDC47
    • CDC47 homolog
    • Cdc47, S. cerevisiae, homolog of
    • DNA replication licensing factor MCM7
    • Homolog of S. cerevisiae Cdc47
    • MCM 2
    • MCM 7
    • MCM2
    • MCM2, formerly
    • Mcm7
    • MCM7 minichromosome maintenance deficient 7
    • MCM7_HUMAN
    • Minichromosome Maintainence 7
    • Minichromosome maintainence, S. cerevisiae, homolog of
    • Minichromosome maintenance complex component 7
    • Minichromosome maintenance deficient 7
    • Minichromosome maintenance protein 7
    • P1.1 MCM3
    • P1.1-MCM3
    • P1CDC47
    • P85MCM
    • PNAS 146
    • PNAS146
    • PPP1R104
    • Protein phosphatase 1, regulatory subunit 104
    see all
  • Function
    Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage.
  • Sequence similarities
    Belongs to the MCM family.
    Contains 1 MCM domain.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus.
  • Information by UniProt

Images

  • ab114356 analysed on a 12.5% SDS-PAGE gel stained with Coomassie Blue.

References

ab114356 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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