Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesHuman
    • SequenceThe sequence of the first five N-terminal amino acids was determined and was found to be Gln-Pro-Val-Gly-Ile.

Specifications

Our Abpromise guarantee covers the use of ab73833 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityBiological Activity: The specific activity as determined by the ability of MCP3 to chemoattract human peripheral blood at 10-100ng/ml.
  • Applications

    SDS-PAGE

    Western blot

  • Purity> 95 % SDS-PAGE.
    Purified by proprietary chromatographic techniques. Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
  • FormLyophilised
  • Additional notesFor long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • ReconstitutionLyophilized from a concentrated (1mg/ml) sterile solution containing no additives. Reconstitute the lyophilized product in sterile 18MOhm-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

General Info

  • Alternative names
    • C-C motif chemokine 7
    • Ccl7
    • CCL7_HUMAN
    • Chemokine CC motif ligand 7
    • FIC
    • MARC
    • MCP-3
    • Monocyte chemoattractant protein 3
    • Monocyte chemotactic protein 3
    • NC28
    • RP23-350G1.4
    • SCYA6
    • SCYA7
    • Small-inducible cytokine A7
    see all
  • FunctionChemotactic factor that attracts monocytes and eosinophils, but not neutrophils. Augments monocyte anti-tumor activity. Also induces the release of gelatinase B. This protein can bind heparin. Binds to CCR1, CCR2 and CCR3.
  • Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
  • Post-translational
    modifications
    O-glycosylated.
  • Cellular localizationSecreted.
  • Information by UniProt

Recombinant human MCP3 protein images

  • All lanes : Anti-MCP3 antibody (ab104866) at 1 µg/ml

    Lane 1 : Recombinant human MCP3 protein (ab73833) at 0.1 µg
    Lane 2 : Recombinant human MCP3 protein (ab73833) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 1 minute

References for Recombinant human MCP3 protein (ab73833)

ab73833 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab73833.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"