Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Species
      Human
    • Sequence
      The sequence of the first five N-terminal amino acids was determined and was found to be Gln-Pro-Val-Gly-Ile.

Specifications

Our Abpromise guarantee covers the use of ab73833 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Biological Activity: The specific activity as determined by the ability of MCP3 to chemoattract human peripheral blood at 10-100ng/ml.
  • Applications

    SDS-PAGE

    Western blot

  • Purity
    > 95 % SDS-PAGE.
    Purified by proprietary chromatographic techniques. Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
  • Form
    Lyophilised
  • Additional notes
    For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Lyophilized from a concentrated (1mg/ml) sterile solution containing no additives. Reconstitute the lyophilized product in sterile 18MOhm-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

General Info

  • Alternative names
    • C-C motif chemokine 7
    • Ccl7
    • CCL7_HUMAN
    • Chemokine CC motif ligand 7
    • FIC
    • MARC
    • MCP-3
    • Monocyte chemoattractant protein 3
    • Monocyte chemotactic protein 3
    • NC28
    • RP23-350G1.4
    • SCYA6
    • SCYA7
    • Small-inducible cytokine A7
    see all
  • Function
    Chemotactic factor that attracts monocytes and eosinophils, but not neutrophils. Augments monocyte anti-tumor activity. Also induces the release of gelatinase B. This protein can bind heparin. Binds to CCR1, CCR2 and CCR3.
  • Sequence similarities
    Belongs to the intercrine beta (chemokine CC) family.
  • Post-translational
    modifications
    O-glycosylated.
  • Cellular localization
    Secreted.
  • Information by UniProt

Images

  • All lanes : Anti-MCP3 antibody (ab104866) at 1 µg/ml

    Lane 1 : Recombinant human MCP3 protein (ab73833) at 0.1 µg
    Lane 2 : Recombinant human MCP3 protein (ab73833) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 1 minute

References

ab73833 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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