Recombinant human MCSF Receptor protein (Fc Chimera) (ab83682)



  • NatureRecombinant
  • SourceHEK 293 cells
  • Amino Acid Sequence
    • AccessionP07333
    • SpeciesHuman
    • Amino acids1 to 512
    • Additional sequence informationFused with the Fc region of Human IgG1 at the C-terminus.


Our Abpromise guarantee covers the use of ab83682 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityActivity: The ED50 of ab83682 is typically 0.2 - 0.3 ug/ml as measured by its ability to neutralize MCSF mediated proliferation of mNFS-60 cells.
  • Applications

    Functional Studies


  • Purity> 95 % SDS-PAGE.

  • FormLyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C.

    Preservative: None
    Constituents: 10% Trehalose, 1% Human serum albumin, PBS

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • ReconstitutionIt is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.

General Info

  • Alternative names
    • C FMS
    • CD 115
    • CD115
    • CD115 antigen
    • CFMS
    • Colony stimulating factor 1 receptor
    • Colony stimulating factor I receptor
    • CSF 1 R
    • CSF 1R
    • CSF-1 receptor
    • CSF-1-R
    • CSF1 R
    • CSF1R
    • CSFR
    • EC
    • FIM 2
    • FIM2
    • FMS
    • FMS proto oncogene
    • FMS protooncogene
    • HDLS
    • M-CSF Receptor
    • M-CSF-R
    • Macrophage colony stimulating factor 1 receptor
    • Macrophage colony stimulating factor I receptor
    • Macrophage colony-stimulating factor 1 receptor
    • McDonough feline sarcoma viral (v fms) oncogene homolog
    • MCSFR
    • Oncogen FMS
    • Proto-oncogene c-Fms
    • V-FMS McDonough feline sarcoma viral oncogen homolog, formerly
    see all
  • FunctionProtein tyrosine-kinase transmembrane receptor for CSF1 and IL34.
  • Tissue specificityExpressed in bone marrow and in differentiated blood mononuclear cells.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Cellular localizationMembrane.
  • Information by UniProt

Recombinant human MCSF Receptor protein (Fc Chimera) images

  • Densitometry of protein isoforms visualised by 2-DE. The triangle indicates the theoretical MW and pI of the protein.
  • 1D SDS-PAGE of ab83682 before and after treatment with glycosidases to remove oligosaccharides.
    Lane 1 MW markers; Lane 2 ab83682; Lane 3 ab83682 treated with PNGase F to remove potential N-linked glycans; Lane 4 ab83682 treated with a glycosidase cocktail to remove potential N- and O-linked glycans. Approximately 5 µg of protein was loaded per lane.

    Drop in MW after treatment with PNGase F indicates presence of N-linked glycans. A further drop in MW after treatment with the glycosidase cocktail indicates the presence of O-linked glycans. Additional bands in lane 3 and lane 4 are glycosidase enzymes.

  • A sample of ab83682 without carrier protein was reduced and alkylated and focused on a 3-10 IPG strip then run on a 4-20% Tris-HCl 2D gel. Approximately 40 µg of protein was load; Gel was stained using Deep Purple™.

References for Recombinant human MCSF Receptor protein (Fc Chimera) (ab83682)

ab83682 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting me.

I received an answer from the lab. The lab confirmed, that for the ab83682 a NuPage 4-20% Tris-Glycine SDS gel was used together with a standard buffer system.

The lab also confirmed, that it is not recomm...

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Thank you very much for providing your WB result.

1. As far as I can see the band detected in samples 5 to 7 are at the correct size between 100-140 kDa. I think the band that shows up at about 70 kda might still be the BSA. Because of the hig...

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Thank you for this new image.

It would be beneficial to have a new western blot result to assure which bands are the MCSF protein. Like mentioned before, I suspect the strong band at about 77 kDa might be BSA, which is a content of the storage...

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Thank you for your email.

Thank you for giving me this further information. I am pleased that you will try the given suggestions for the experiment. I hope this might be helpful for you.

I can confirm that I think there are bands at t...

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I apologize for making you wait this long. Regrettably I did not receive a confirmation about the used gel from the lab. I wrote them a reminder email as you did with me. Thank you very much for this.

Meanwhile I was looking through the data y...

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I like to reassure you that we have not forgotten about your inquiry. Anja is currently out of office. However, I know about your inquiry and I will get back to you as soon as I receive an answer from the lab. Regrettably I have not received the needed...

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