• NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesHuman
    • Amino acids1 to 491
    • TagsHis tag C-Terminus , His tag N-Terminus
    • Additional sequence informationThis protein has a 6X His tag on its N-Terminus and a 8X His tag on its C-Terminus


Our Abpromise guarantee covers the use of ab82080 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity1 unit equals 1 nanogram of purified protein.
  • Applications


  • FormLiquid
  • Additional notes1 unit equals 1 nanogram of purified protein.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 20% Glycerol, 20mM Tris Cl, 100mM Potassium chloride, 1mM DTT, 0.2mM EDTA, pH 8.0

General Info

  • Alternative names
    • ACTFS
    • Double minute 2 protein
    • E3 ubiquitin-protein ligase Mdm2
    • Hdm 2
    • Hdm2
    • HDMX
    • MDM 2
    • MDM2
    • MDM2 oncogene E3 ubiquitin protein ligase
    • Mdm2 p53 E3 ubiquitin protein ligase homolog
    • Mdm2 transformed 3T3 cell double minute 2 p53 binding protein (mouse) binding protein 104kDa
    • MDM2_HUMAN
    • MDM2BP
    • Mouse Double Minute 2
    • MTBP
    • Murine Double Minute Chromosome 2
    • Oncoprotein Mdm2
    • p53 Binding Protein Mdm2
    • p53-binding protein Mdm2
    • Ubiquitin protein ligase E3 Mdm2
    see all
  • FunctionE3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degradation by the proteasome. Inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Also acts as an ubiquitin ligase E3 toward itself and ARRB1. Permits the nuclear export of p53/TP53. Promotes proteasome-dependent ubiquitin-independent degradation of retinoblastoma RB1 protein. Inhibits DAXX-mediated apoptosis by inducing its ubiquitination and degradation. Component of the TRIM28/KAP1-MDM2-p53/TP53 complex involved in stabilizing p53/TP53. Also component of the TRIM28/KAP1-ERBB4-MDM2 complex which links growth factor and DNA damage response pathways.
  • Tissue specificityUbiquitous. Isoform Mdm2-A, isoform Mdm2-B, isoform Mdm2-C, isoform Mdm2-D, isoform Mdm2-E, isoform Mdm2-F and isoform Mdm2-G are observed in a range of cancers but absent in normal tissues.
  • Involvement in diseaseNote=Seems to be amplified in certain tumors (including soft tissue sarcomas, osteosarcomas and gliomas). A higher frequency of splice variants lacking p53 binding domain sequences was found in late-stage and high-grade ovarian and bladder carcinomas. Four of the splice variants show loss of p53 binding.
  • Sequence similaritiesBelongs to the MDM2/MDM4 family.
    Contains 1 RanBP2-type zinc finger.
    Contains 1 RING-type zinc finger.
    Contains 1 SWIB domain.
  • DomainRegion I is sufficient for binding p53 and inhibiting its G1 arrest and apoptosis functions. It also binds p73 and E2F1. Region II contains most of a central acidic region required for interaction with ribosomal protein L5 and a putative C4-type zinc finger. The RING finger domain which coordinates two molecules of zinc interacts specifically with RNA whether or not zinc is present and mediates the heterooligomerization with MDM4. It is also essential for its ubiquitin ligase E3 activity toward p53 and itself.
  • Post-translational
    Phosphorylated in response to ionizing radiation in an ATM-dependent manner.
    Auto-ubiquitinated; which leads to proteasomal degradation. Deubiquitinated by USP2 leads to its accumulation and increases deubiquitinilation and degradation of p53/TP53. Deubiquitinated by USP7; leading to stabilize it.
  • Cellular localizationNucleus > nucleoplasm. Cytoplasm. Nucleus > nucleolus. Expressed predominantly in the nucleoplasm. Interaction with ARF(P14) results in the localization of both proteins to the nucleolus. The nucleolar localization signals in both ARF(P14) and MDM2 may be necessary to allow efficient nucleolar localization of both proteins. Colocalizes with RASSF1 isoform A in the nucleus.
  • Information by UniProt

References for Recombinant Human MDM2 protein (ab82080)

This product has been referenced in:
  • Elshafey R  et al. Label-free impedimetric immunosensor for ultrasensitive detection of cancer marker Murine double minute 2 in brain tissue. Biosens Bioelectron 39:220-5 (2013). Read more (PubMed: 22898660) »

See 1 Publication for this product

Product Wall

The sequence for the MDM 2 protein (His tag) ab82080 is as follows:


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I just went through our database and we do nothave any experimental data about the ligase activity ab82080 (MDM2).

This protein together with p53 wt has beenused byseveral pharma companies and academic la...

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Thank you for your inquiry, and for your patience while I looked into this. The MDM2 protein has a 6x His tag on its N-terminus and 8 x Histag on its C-terminus. The protein sequence is given below: MAHHHHHHAS MCNTNMSVPTDGAVTTSQIPASEQETLVRPK...

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I am very pleased to hear you would like to accept our offer and test ab82080 in functional studies involving protein-protein interactions. This code will give you 1 free protein before the expiration date. To redeem this offer, please submit an Abrevi...

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