The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
> 250.000 U/mg (1 Unit is defined as the amount of MEK1 which activates unactive ERK1 (0.3 mg/ml) by 1 U/min using 100 µM ATP at 30°C. 1 U ERK1 activity is defined as 1 pmol phosphate transferred to myelin basic protein (0.2 mg/ml) per min using 125 µM ATP at 30°C).
% SDS-PAGE. ab90031 is purified by gluthatione sepharose and gel filtration.
MEK1 can be activated by Raf by phosphorylation on serine 218 and serine 222. Mutation of these sites to acidic residues leads to constitutively active MEK1 in some cases.
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Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Dual specificity mitogen activated protein kinase kinase 1
Dual specificity mitogen-activated protein kinase kinase 1
ERK activator kinase 1
MAP kinase kinase 1
MAP kinase/Erk kinase 1
MAPK/ERK kinase 1
Mitogen activated protein kinase kinase 1
Protein kinase mitogen activated kinase 1 (MAP kinase kinase 1)
Protein kinase mitogen activated, kinase 1
FunctionCatalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.
Tissue specificityWidely expressed, with extremely low levels in brain.
Involvement in diseaseDefects in MAP2K1 are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. Contains 1 protein kinase domain.
Post-translational modificationsPhosphorylation on Ser/Thr by MAP kinase kinase kinases (RAF or MEKK1) regulates positively the kinase activity. Acetylation by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.