Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      MGSSHHHHHHSSGLVPRGSHMGSHMLARRKPVLPALTINPTIAEGPSPTS EGASEANLVDLQKKLEELELDEQQKKRLEAFLTQKAKVGELKDDDFERIS ELGAGNGGVVTKVQHRPSGLIMARKLIHLEIKPAIRNQIIRELQVLHECN SPYIVGFYGAFYSDGEISICMEHMDGGSLDQVLKEAKRIPEEILGKVSIA VLRGLAYLREKHQIMHRDVKPSNILVNSRGEIKLCDFGVSGQLIDSMANS FVGTRSYMAPERLQGTHYSVQSDIWSMGLSLVELAVGRYPIPPPDAKELE AIFGRPVVDGEEGEPHSISPRPRPPGRPVSGHGMDSRPAMAIFELLDYIV NEPPPKLPNGVFTPDFQEFVNKCLIKNPAERADLKMLTNHTFIKRSEVEE VDFAGWLCKTLRLNQPGTPTRTAV
    • Molecular weight
      47 kDa including tags
    • Amino acids
      1 to 400
    • Tags
      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab124543 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Mass Spectrometry

  • Mass spectrometry
    MALDI-TOF
  • Purity
    > 90 % SDS-PAGE.
    ab124543 was purified using conventional chromatography techniques.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.32% Tris HCl, 10% Glycerol, 0.58% Sodium chloride

General Info

  • Alternative names
    • Cardiofaciocutaneous syndrome
    • CFC syndrome
    • CFC4
    • Dual specificity mitogen activated protein kinase kinase 2
    • Dual specificity mitogen-activated protein kinase kinase 2
    • ERK activator kinase 2
    • FLJ26075
    • MAP kinase kinase 2
    • map2k2
    • MAPK / ERK kinase 2
    • MAPK/ERK kinase 2
    • MAPKK 2
    • MAPKK2
    • MEK 2
    • MEK2
    • Microtubule associated protein kinase kinase 2
    • Mitogen activated protein kinase kinase 2
    • Mitogen activated protein kinase kinase 2 p45
    • MKK 2
    • MKK2
    • MP2K2_HUMAN
    • OTTHUMP00000165826
    • OTTHUMP00000165827
    • PRKMK 2
    • PRKMK2
    see all
  • Function
    Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases.
  • Involvement in disease
    Defects in MAP2K2 are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.
  • Sequence similarities
    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1).
    Acetylation of Ser-222 and Ser-226 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
  • Information by UniProt

Images

  • 15% SDS-PAGE analysis of 3 µg ab124543.

References

ab124543 has not yet been referenced specifically in any publications.

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