Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionP46734
    • SpeciesHuman
    • SequenceMGSSHHHHHH SSGLVPRGSH MSKPPAPNPT PPRNLDSRTF ITIGDRNFEV EADDLVTISE LGRGAYGVVE KVRHAQSGTI MAVKRIRATV NSQEQKRLLM DLDINMRTVD CFYTVTFYGA LFREGDVWIC MELMDTSLDK FYRKVLDKNM TIPEDILGEI AVSIVRALEH LHSKLSVIHR DVKPSNVLIN KEGHVKMCDF GISGYLVDSV AKTMDAGCKP YMAPERINPE LNQKGYNVKS DVWSLGITMI EMAILRFPYE SWGTPFQQLK QVVEEPSPQL PADRFSPEFV DFTAQCLRKN PAERMSYLEL MEHPFFTLHK TKKTDIAAFV KEILGEDS
    • Molecular weight38 kDa including tags
    • Amino acids20 to 347
    • TagsHis tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab105578 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Mass Spectrometry

  • Mass spectrometry
    MALDI-TOF
  • Purity> 90 % SDS-PAGE.
    ab105578 was purified using conventional chromatography.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 10% Glycerol, 20mM Tris HCl, pH 8

General Info

  • Alternative names
    • AW212142
    • dual specificity mitogen activated protein kinase kinase 3
    • Dual specificity mitogen-activated protein kinase kinase 3
    • MAP kinase kinase 3
    • map2k3
    • MAPK ERK kinase 3
    • MAPK/ERK kinase 3
    • MAPKK 3
    • MAPKK3
    • MEK 3
    • MEK3
    • Mitogen activated protein kinase kinase 3
    • MKK 3
    • MKK3
    • mMKK3b
    • MP2K3_HUMAN
    • PRKMK 3
    • PRKMK3
    • protein kinase, mitogen-activated, kinase 3
    • SAPK kinase 2
    • SAPKK 2
    • SAPKK2
    • Stress activated protein kinase kinase 2
    see all
  • FunctionDual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38.
  • Tissue specificityAbundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.
  • Involvement in diseaseNote=Defects in MAP2K3 may be involved in colon cancer.
  • Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated.
    Phosphorylation on Ser-218 and Thr-222 by MAP kinase kinase kinases regulates positively the kinase activity.
    Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway.
  • Information by UniProt

Recombinant Human MEK3 protein images

  • 15% SDS-PAGE analysis of ab105578 (3ug)

References for Recombinant Human MEK3 protein (ab105578)

ab105578 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab105578.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"