The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Additional notesProtein concentration is above or equal to 0.05 mg/ml. ab114459 is best used within three months from the date of receipt.
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Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00 Constituents: 0.79% Tris HCl, 0.3% Glutathione
Dual specificity mitogen activated protein kinase kinase 5
Dual specificity mitogen-activated protein kinase kinase 5
MAP kinase kinase 5
MAP kinase kinase MEK5b
MAPK/ERK kinase 5
mitogen-activated protein kinase kinase 5
Protein kinase, mitogen-activated, kinase 5
FunctionActs as a scaffold for the formation of a ternary MAP3K2/MAP3K3-MAP3K5-MAPK7 signaling complex. Activation of this pathway appear to play a critical role in protecting cells from stress-induced apopotosis, neuronal survival and cardiac development and angiogenesis.
Tissue specificityExpressed in many adult tissues. Abundant in heart and skeletal muscle.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. Contains 1 OPR domain. Contains 1 protein kinase domain.
DomainBinds MAP3K2/MAP3K3 and MAPK7 via non-overlapping residues of the OPR domain. This domain also mediates interactions with SQSTM1 and PARD6A.
Post-translational modificationsActivated by phosphorylation on Ser/Thr by MAP kinase kinase kinases. Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway.