Overview

Description

  • NatureRecombinant
  • SourceBaculovirus
  • Amino Acid Sequence
    • SpeciesHuman
    • Amino acids2 to 334

Specifications

Our Abpromise guarantee covers the use of ab95252 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityEnzyme reaction is conducted in a buffer containing 50 mM HEPES (pH7.5), 10 mM MgCl2, 1 mM EGTA, 200 µM ATP, 0.01% Brij-35, 100ng of inactive MAPK14 and 2 µM substrate (SER/THR 4) at room temperature for 1 hour. Developer solution was added to reaction and reaction was stopped after 1h of incubation at RT.
  • Applications

    Functional Studies

    SDS-PAGE

  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 20% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 8.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • Dual specificity mitogen activated protein kinase kinase 6
    • Dual specificity mitogen-activated protein kinase kinase 6
    • MAP kinase kinase 6
    • MAP2K6
    • MAPK/ERK kinase 6
    • MAPKK 6
    • MAPKK6
    • MEK 6
    • MEK6
    • Mitogen Activated Protein Kinase Kinase 6
    • MKK 6
    • MKK6
    • MP2K6_HUMAN
    • PRKMK6
    • Protein kinase mitogen activated kinase 6
    • protein kinase, mitogen-activated, kinase 6 (MAP kinase kinase 6)
    • SAPK kinase 3
    • SAPKK-3
    • SAPKK3
    • SKK3
    • Stress-activated protein kinase kinase 3
    see all
  • FunctionCatalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in MAP kinase p38 exclusively.
  • Tissue specificityIsoform 2 is only expressed in skeletal muscle. Isoform 1 is expressed in skeletal muscle, heart, and in lesser extent in liver or pancreas.
  • Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Weakly autophosphorylated.
    Acetylation of Ser-207 and Thr-211 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
  • Information by UniProt

Recombinant human MEK6 protein images

  • SDS-PAGE showing ab95252 at approximately 63.5kDa (5µg). Lane 2 shows molecular weight markers.
  • Image showing specific activity of ab95252.

References for Recombinant human MEK6 protein (ab95252)

ab95252 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab95252.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"