Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus
  • Amino Acid Sequence
    • Species
      Human
    • Amino acids
      2 to 334

Specifications

Our Abpromise guarantee covers the use of ab95252 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH7.5), 10 mM MgCl2, 1 mM EGTA, 200 µM ATP, 0.01% Brij-35, 100ng of inactive MAPK14 and 2 µM substrate (SER/THR 4) at room temperature for 1 hour. Developer solution was added to reaction and reaction was stopped after 1h of incubation at RT.
  • Applications

    Functional Studies

    SDS-PAGE

  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 20% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 8.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • Dual specificity mitogen activated protein kinase kinase 6
    • Dual specificity mitogen-activated protein kinase kinase 6
    • MAP kinase kinase 6
    • MAP2K6
    • MAPK/ERK kinase 6
    • MAPKK 6
    • MAPKK6
    • MEK 6
    • MEK6
    • Mitogen Activated Protein Kinase Kinase 6
    • MKK 6
    • MKK6
    • MP2K6_HUMAN
    • PRKMK6
    • Protein kinase mitogen activated kinase 6
    • protein kinase, mitogen-activated, kinase 6 (MAP kinase kinase 6)
    • SAPK kinase 3
    • SAPKK-3
    • SAPKK3
    • SKK3
    • Stress-activated protein kinase kinase 3
    see all
  • Function
    Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in MAP kinase p38 exclusively.
  • Tissue specificity
    Isoform 2 is only expressed in skeletal muscle. Isoform 1 is expressed in skeletal muscle, heart, and in lesser extent in liver or pancreas.
  • Sequence similarities
    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Weakly autophosphorylated.
    Acetylation of Ser-207 and Thr-211 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
  • Information by UniProt

Images

  • SDS-PAGE showing ab95252 at approximately 63.5kDa (5µg). Lane 2 shows molecular weight markers.
  • Image showing specific activity of ab95252.

References

ab95252 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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