Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Molecular weight
      155 kDa including tags
    • Amino acids
      664 to 1512

Specifications

Our Abpromise guarantee covers the use of ab104027 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    The Specific activity of ab104027 was determined to be 820 nmol/min/mg.
  • Applications

    SDS-PAGE

    Functional Studies

  • Purity
    > 75 % SDS-PAGE.
    Purity was determined to be >75% by densitometry.
  • Form
    Liquid
  • Additional notes

    ab126919 (Human MEK1 full length protein), ab126906 (Human ERK1 full length protein) and ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity

     

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 0.01% Triton-X-100, 50mM Tris HCl, 150mM Sodium chloride, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • M3K1_HUMAN
    • MAP3K1
    • MAPK/ERK kinase kinase 1
    • MAPKKK1
    • MEK kinase 1
    • Mekk
    • MEKK 1
    • MEKK1
    • Mitogen activated protein kinase kinase kinase 1
    • Mitogen activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase
    • Mitogen-activated protein kinase kinase kinase 1
    • SRXY6
    see all
  • Function
    Component of a protein kinase signal transduction cascade. Activates the ERK and JNK kinase pathways by phosphorylation of MAP2K1 and MAP2K4. Activates CHUK and IKBKB, the central protein kinases of the NF-kappa-B pathway.
  • Sequence similarities
    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 protein kinase domain.
    Contains 1 RING-type zinc finger.
    Contains 1 SWIM-type zinc finger.
  • Post-translational
    modifications
    Autophosphorylated.
  • Information by UniProt

Images

  • Kinase Assay demonstrating specific activity of ab104027.
  • SDS-PAGE showing ab104027 at approximately 155kDa.

References

ab104027 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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