The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Endotoxin level< 0.100 Eu/µg
% SDS-PAGE. Greater than 98% by SDS-PAGE and HPLC analyses.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Endotoxin level is less than 0.1 ng per µg (1EU/µg).
ReconstitutionCentrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4oC for 1 week or -20oC for future use.
Cartilage derived retinoic acid sensitive protein
Melanoma derived growth regulatory protein precursor
Melanoma inhibitory activity protein
Melanoma-derived growth regulatory protein
FunctionElicits growth inhibition on melanoma cells in vitro as well as some other neuroectodermal tumors, including gliomas.
Tissue specificityAll malignant melanoma cell lines tested and infrequently in glioma cell lines.
Sequence similaritiesBelongs to the MIA/OTOR family. Contains 1 SH3 domain.
Post-translational modificationsMay possess two intramolecular disulfide bonds.