Recombinant human MELK (mutated T460 M) protein (ab179957)

Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      MKDYDELLKYYELHETIGTGGFAKVKLACHILTGEMVAIKIMDKNTLGSD LPRIKTEIEALKNLRHQHICQLYHVLETANKIFMVLEYCPGGELFDYIIS QDRLSEEETRVVFRQIVSAVAYVHSQGYAHRDLKPENLLFDEYHKLKLID FGLCAKPKGNKDYHLQTCCGSLAYAAPELIQGKSYLGSEADVWSMGILLY VLMCGFLPFDDDNVMALYKKIMRGKYDVPKWLSPSSILLLQQMLQVDPKK RISMKNLLNHPWIMQDYNYPVEWQSKNPFIHLDDDCVTELSVHHRNNRQT MEDLISLWQYDHLTATYLLLLAKKARGKPVRLRLSSFSCGQASATPFTDI KSNNWSLEDVTASDKNYVAGLIDYDWCEDDLSTGAATPRTSQFTKYWTES NGVESKSLTPALCRTPANKLKNKENVYTPKSAVKNEEYFMFPEPKTPVNK NQHKREILTMPNRYTTPSKARNQCLKETPIKIPVNSTGTDKLMTGVISPE RRCRSVELDLNQAHMEETPKRKGAKVFGSLERGLDKVITVLTRSKRKGSA
    • Molecular weight
      88 kDa including tags
    • Amino acids
      1 to 550
    • Modifications
      mutated T460 M
    • Tags
      proprietary tag N-Terminus
    • Additional sequence information
      NM_014791

Associated products

Specifications

Our Abpromise guarantee covers the use of ab179957 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    The specific activity of ab179957 was determined to be 120 nmol/min/mg.

  • Applications

    SDS-PAGE

    Functional Studies

  • Purity
    > 80 % Densitometry.

  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 7.50
    Constituents: 0.79% Tris HCl, 25% Glycerol, 0.31% Glutathione, 0.003% EDTA, 0.004% DTT, 0.88% Sodium chloride, 0.002% PMSF

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • AI327312
    • hMELK
    • HPK 38
    • hPK38
    • KIAA0175
    • Likely ortholog of maternal embryonic leucine zipper kinase
    • Maternal embryonic leucine zipper kinase
    • MELK
    • MELK_HUMAN
    • mKIAA0175
    • MPK38
    • OTTHUMP00000021377
    • OTTHUMP00000046113
    • pEg3 kinase
    • Protein kinase Eg3
    • Protein kinase PK38
    • RP23 382O11.1
    • Tyrosine protein kinase MELK
    see all
  • Function
    Phosphorylates ZNF622 and may contribute to its redirection to the nucleus. May be involved in the inhibition of spliceosome assembly during mitosis.
  • Tissue specificity
    Expressed in placenta, kidney, thymus, testis, ovary and intestine.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
    Contains 1 KA1 (kinase-associated) domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated. Thr-478 phosphorylation during mitosis promotes interaction with PPP1R8.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt

Images

  • Sample Kinase Activity Plot.

    The specific activity of ab179957 was determined to be 120 nmol/min/mg.

  • SDS-PAGE analysis of ab179957.

References

ab179957 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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