Overview

Description

  • NatureRecombinant
  • SourceBaculovirus infected Sf9 cells
  • Amino Acid Sequence
    • AccessionQ14680
    • SpeciesHuman
    • Molecular weight61 kDa including tags
    • Amino acids1 to 340

Associated products

Specifications

Our Abpromise guarantee covers the use of ab102551 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityThe Specific activity of ab102551 was determined to be 200 nmol/min/mg.
  • Applications

    Functional Studies

    SDS-PAGE

    Western blot

  • Purity> 85 % Densitometry.
    Purity was determined to be >85% by densitometry.
  • FormLiquid
  • Additional notes

    ab204885 (ZIP Kinase peptide substrate) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • AI327312
    • hMELK
    • HPK 38
    • hPK38
    • KIAA0175
    • Likely ortholog of maternal embryonic leucine zipper kinase
    • Maternal embryonic leucine zipper kinase
    • MELK
    • MELK_HUMAN
    • mKIAA0175
    • MPK38
    • OTTHUMP00000021377
    • OTTHUMP00000046113
    • pEg3 kinase
    • Protein kinase Eg3
    • Protein kinase PK38
    • RP23 382O11.1
    • Tyrosine protein kinase MELK
    see all
  • FunctionPhosphorylates ZNF622 and may contribute to its redirection to the nucleus. May be involved in the inhibition of spliceosome assembly during mitosis.
  • Tissue specificityExpressed in placenta, kidney, thymus, testis, ovary and intestine.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
    Contains 1 KA1 (kinase-associated) domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated. Thr-478 phosphorylation during mitosis promotes interaction with PPP1R8.
  • Cellular localizationCytoplasm.
  • Information by UniProt

Recombinant human MELK protein images

  • The Specific activity of ab102551 was determined to be 200 nmol/min/mg.
  • SDS-PAGE showing ab102551 at approximately 61kDa.

References for Recombinant human MELK protein (ab102551)

ab102551 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"