Recombinant Human MESDC2 protein (ab104671)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      MGSSHHHHHHSSGLVPRGSHMAEGSPGTPDESTPPPRKKKKDIRDYNDAD MARLLEQWEKDDDIEEGDLPEHKRPSAPVDFSKIDPSKPESILKMTKKGK TLMMFVTVSGSPTEKETEEITSLWQGSLFNANYDVQRFIVGSDRAIFMLR DGSYAWEIKDFLVGQDRCADVTLEGQVYPGKGGGSKEKNKTKQDKGKKKK EGDLKSRSSKEENRAGNKREDL
    • Molecular weight
      25 kDa including tags
    • Amino acids
      34 to 234
    • Tags
      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab104671 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Mass Spectrometry

  • Mass spectrometry
    MALDI-TOF
  • Purity
    > 90 % SDS-PAGE.
    ab104671 is purified by using anion-exchange chromatography (DEAE sepharose resin) and gel-filtration chromatography (Sephacryl S-200) with 20mM Tris pH 7.5, 2mM EDTA.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 20% Glycerol, 0.1M Sodium chloride, 20mM Tris HCl, 1mM DTT, pH 8.0

General Info

  • Alternative names
    • BOCA
    • KIAA0081
    • LDLR chaperone MESD
    • MESD
    • MESD_HUMAN
    • MESDC 2
    • mesdc2
    • Mesoderm development candidate 2
    • Mesoderm development protein
    • Renal carcinoma antigen NY REN 61
    • Renal carcinoma antigen NY-REN-61
    see all
  • Function
    Chaperone specifically assisting the folding of beta-propeller/EGF modules within the family of low-density lipoprotein receptors (LDLRs). Acts as a modulator of the Wnt pathway through chaperoning the coreceptors of the canonical Wnt pathway, LRP5 and LRP6, to the plasma membrane. Essential for specification of embryonic polarity and mesoderm induction.
  • Sequence similarities
    Belongs to the MESD family.
  • Domain
    The chaperone domain provides a folding template for proper folding of the beta-propeller (BP) domains of LRP5/6.
    The escort domain ensures LRP5/6 safe-trafficking from the ER to the Golgi by preventing premature ligand-binding.
  • Cellular localization
    Endoplasmic reticulum.
  • Information by UniProt

Recombinant Human MESDC2 protein images

  • 15% SDS-PAGE showing ab104671 at approximately 24.9kDa (3µg).
    Note: Real molecular weight on SDS-PAGE is shifted up compared to predicted molecular weight.

References for Recombinant Human MESDC2 protein (ab104671)

ab104671 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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