Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Species
      Human
    • Sequence
      MGSSHHHHHH SSGLVPRGSH MDKDCEMKRT TLDSPLGKLE LSGCEQGLHE IKLLGKGTSA ADAVEVPAPA AVLGGPEPLM QCTAWLNAYF HQPEAIEEFP VPAFHHPVFQ QESFTRQVLW KLLKVVKFGE VISYQQLAAL AGNPKAARAV GGAMRGNPVP ILIPCHRVVC SSGAVGNYSG GLAVKEWLLA HEGHRLGKPG LGGSSGLAGA WLKGAGATSG SPPAGRN

Specifications

Our Abpromise guarantee covers the use of ab79251 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

  • Purity
    > 95 % SDS-PAGE.
    ab79251 is purified using conventional chromatography techniques. Endotoxin Level:< 1.0 EU per 1 µg of protein (determined by LAL method)
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Glycerol, 20mM Tris HCl, 1mM DTT, pH 7.5

General Info

  • Alternative names
    • 6 O methylguanine DNA methyltransferase
    • 6-O-methylguanine-DNA methyltransferase
    • Agat
    • AGT
    • AI267024
    • EC 2.1.1.63
    • Methylated DNA protein cysteine methyltransferase
    • Methylated-DNA--protein-cysteine methyltransferase
    • Methylguanine DNA methyltransferase
    • MGC107020
    • MGMT
    • MGMT_HUMAN
    • O 6 methylguanine DNA alkyltransferase
    • O 6 methylguanine DNA methyltransferase
    • O-6-methylguanine-DNA methyltransferase
    • O-6-methylguanine-DNA-alkyltransferase
    see all
  • Function
    Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
  • Sequence similarities
    Belongs to the MGMT family.
  • Cellular localization
    Nucleus.
  • Information by UniProt

References for Recombinant Human MGMT protein (ab79251)

ab79251 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab79251.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up