The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Additional notesProtein concentration is above or equal to 0.05 µg/ul. Best use within three months from the date of receipt of this protein.
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Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00 Constituents: 0.3% Glutathione, 0.79% Tris HCl
E3 ubiquitin-protein ligase Midline-1
Finger on X and Y mouse homolog of
Midline 1 (Opitz/BBB syndrome)
Midline 1 ring finger
Midline 1 RING finger protein
Putative transcription factor XPRF
RING finger protein 59
RING finger protein Midline-1
Tripartite motif containing protein 18
Tripartite motif protein TRIM18
Tripartite motif-containing protein 18
zinc finger on X and Y, mouse, homolog of
Zinc finger X and Y
FunctionMay have E3 ubiquitin ligase activity which targets the catalytic subunit of protein phosphatase 2 for degradation.
Tissue specificityIn the fetus, highest expression found in kidney, followed by brain and lung. Expressed at low levels in fetal liver. In the adult, most abundant in heart, placenta and brain.
Involvement in diseaseDefects in MID1 are the cause of Opitz syndrome type I (OS-I) [MIM:300000]. OS-I is an X-linked recessive disorder characterized by hypertelorism, genital-urinary defects such as hypospadias in males and splayed labia in females, lip-palate-laryngotracheal clefts, imperforate anus, developmental delay and congenital heart defects. OS-I mutations produce proteins with a decreased affinity for microtubules.
Sequence similaritiesBelongs to the TRIM/RBCC family. Contains 2 B box-type zinc fingers. Contains 1 B30.2/SPRY domain. Contains 1 COS domain. Contains 1 fibronectin type-III domain. Contains 1 RING-type zinc finger.
Post-translational modificationsPhosphorylated on serine and threonine residues.
Cellular localizationCytoplasm. Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > spindle. Microtubule-associated. It is associated with microtubules throughout the cell cycle, co-localizing with cytoplasmic fibers in interphase and with the mitotic spindle and midbodies during mitosis and cytokinesis.