Recombinant Human Midkine protein (ab73234)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Species
      Human
    • Sequence
      MKHHHHHHHM KKKDKVKKGG PGSECAEWAW GPCTPSSKDC GVGFREGTCG AQTQRIRCRV PCNWKKEFGA DCKYKFENWG ACDGGTGTKV RQGTLKKARY NAQCQETIRV TKPCTPKTKA KAKAKKGKGK D
    • Tags
      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab73234 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    ELISA

    SDS-PAGE

  • Purity
    > 95 % SDS-PAGE.
    ab73234 is purified by a three-step procedure using affinity Ni-NTA chromatography and size exclusion chromatography before and after refolding.
  • Form
    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 0.1M Sodium chloride, 0.05M PBS, pH 7.2

  • Reconstitution
    To reconstitute: Add 0.2 ml of PBS pH 7.2 and let the lyophilized pellet dissolve completely. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles.

General Info

  • Alternative names
    • Amphiregulin associated protein
    • Amphiregulin-associated protein
    • ARAP
    • FLJ27379
    • Mdk
    • Midgestation and kidney protein
    • Midkine
    • MK
    • MK 1
    • MK_HUMAN
    • MK1
    • NEGF 2
    • NEGF2
    • Neurite growth promoting factor 2
    • Neurite outgrowth promoting protein
    • Neurite outgrowth-promoting factor 2
    • Neurite outgrowth-promoting protein
    • Retinoic acid inducible factor
    see all
  • Function
    Has heparin binding activity, and growth promoting activity. Involved in neointima formation after arterial injury, possibly by mediating leukocyte recruitment. Also involved in early fetal adrenal gland development.
  • Sequence similarities
    Belongs to the pleiotrophin family.
  • Cellular localization
    Secreted.
  • Information by UniProt

References

ab73234 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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