Recombinant human MIF protein (ab75432)

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Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Species
      Human
    • Sequence
      MPMFIVNTNV PRASVPDGFL SELTQQLAQA TGKPPQYIAV HVVPDQLMAF GGSSEPCALC SLHSIGKIGG AQNRSYSKLL CGLLAERLRI SPDRVYINYY DMNAANVGWN NSTFA

Specifications

Our Abpromise guarantee covers the use of ab75432 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

  • Purity
    > 95 % SDS-PAGE.
    Purity is approximately 97% as determined by analysis by RP-HPLC and reducing and non-reducing SDS-PAGE. Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
  • Form
    Lyophilised
  • Additional notes


    ab75432 acts as an inflammatory mediator consisting of two alpha-helices and six beta-strands, four of which form a beta-sheet structure. The remaining two beta-strands interact with other MIF molecules forming a trimer in solution. MIF acts on fibroblasts by inducing IL-1, IL-8 and MMP expression. MIF stimulates NO production and TNF-alpha release following IFN-gamma activation on macrophages.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 10mM Sodium phosphate, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Reconstitute with sterile water at 0.1 - 1.0 mg/ml, which can then be further diluted to other aqueous solutions.

General Info

  • Alternative names
    • GIF
    • GLIF
    • Glycosylation inhibiting factor
    • Glycosylation-inhibiting factor
    • L-dopachrome isomerase
    • L-dopachrome tautomerase
    • Macrophage migration inhibitory factor
    • Macrophage migration inhibitory factor (glycosylation-inhibiting factor)
    • MIF
    • MIF protein
    • MIF_HUMAN
    • MMIF
    • Phenylpyruvate tautomerase
    see all
  • Function
    Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.
  • Involvement in disease
    Genetic variations in MIF are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
  • Sequence similarities
    Belongs to the MIF family.
  • Cellular localization
    Secreted. Cytoplasm. Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens.
  • Information by UniProt

Recombinant human MIF protein images

  • Other-MIF protein (Active)(ab75432)
    Other-MIF protein (Active)(ab75432)
    ab75432 used in Western Blot. Figure: 1 ug run under (+) reducing and (-) non-reducing conditions in a 4-20% Tris-Glycine gel stained with Coomassie Blue. Human MIF has a predicted MW of 12.5 kDa.

References for Recombinant human MIF protein (ab75432)

ab75432 has not yet been referenced specifically in any publications.

Publishing research using ab75432? Please let us know so that we can cite the reference in this datasheet.

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Submit an abreview for ab75432 Submit a question for ab75432

1 Abreview or Q&A

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1) Was sind die zusaetzlichen Reste (MIF 115aa)? 2) ist es damit fuer funktionelle Studien geeignet? oder ist ab73230 oder ab75432 besser geeignet?

Vielen Dank für Ihre Anrufe. Bezüglich des His-getagten MIF-Proteins ab51096 kann ich Ihnen mitteilen, dass die ersten 36 Aminosäuren des Konstrukts den His-tag sowie weiteren Tag und eine Schnittstelle beinhalten, wodurch sich die Gesamtlänge von 151 ...

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