Recombinant human Mitochondrial Pyruvate dehydrogenase kinase 1 protein (ab110359)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionQ15118
    • SpeciesHuman
    • Amino acids29 to 436
    • TagsHis tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab110359 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Sandwich ELISA

    Functional Studies

  • Purity> 90 % Purified via His tag.

  • FormLiquid
  • Additional notes

     

    PDH Kinases (PDKs) deactivate PDH by reversible phosphorylation. In humans and rodents, 4 PDK's are present in an organ-dependent manner. In all cases, PDK's are physically part of the PDH complex (binding via lipoyl domain 2 of E2), and they phosphorylate the E1 subunit at 3 specific serine residues. PDH phosphatases remove the phosphorylation and restore activity of PDH.

    Product was previously marketed under the MitoSciences sub-brand.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.14% Sodium phosphate, 1.46% Sodium chloride, 30% Glycerol, 0.08% DTT, 0.02% EDTA, 0.05% Triton-X-100

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial
    • HGNC:8809
    • Mitochondrial pyruvate dehydrogenase kinase isoenzyme 1
    • PDH kinase 1
    • Pdk1
    • PDK1_HUMAN
    • Pyruvate dehydrogenase kinase isoform 1
    • Pyruvate dehydrogenase kinase, isoenzyme 1
    see all
  • FunctionInhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
  • Tissue specificityExpressed predominantly in the heart.
  • Sequence similaritiesBelongs to the PDK/BCKDK protein kinase family.
    Contains 1 histidine kinase domain.
  • Cellular localizationMitochondrion matrix.
  • Information by UniProt

References for Recombinant human Mitochondrial Pyruvate dehydrogenase kinase 1 protein (ab110359)

ab110359 has not yet been referenced specifically in any publications.

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Thank you for your enquiry. This is an interesting question and I would be pleased to help you further.  In order to do so, I would appreciate if you could confirm the following: 1. Are you wanting to measure viability? Or metabolic activity? Or...

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