Recombinant human Mitochondrial Pyruvate dehydrogenase kinase 1 protein (ab125580)

Overview

Description

  • NatureRecombinant
  • SourceBaculovirus infected Sf9 cells
  • Amino Acid Sequence
    • AccessionQ15118
    • SpeciesHuman
    • Molecular weight70 kDa including tags
    • Amino acids1 to 436

Specifications

Our Abpromise guarantee covers the use of ab125580 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityThe specific activity of ab125580 was determined to be 1.7 nmol/min/mg
  • Applications

    Western blot

    Functional Studies

    SDS-PAGE

  • FormLiquid
  • Additional notes

    ab125602 (Human Pyruvate Dehydrogenase E1-alpha subunit full length protein) can be utilized as a substrate for assessing Kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.88% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial
    • HGNC:8809
    • Mitochondrial pyruvate dehydrogenase kinase isoenzyme 1
    • PDH kinase 1
    • Pdk1
    • PDK1_HUMAN
    • Pyruvate dehydrogenase kinase isoform 1
    • Pyruvate dehydrogenase kinase, isoenzyme 1
    see all
  • FunctionInhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
  • Tissue specificityExpressed predominantly in the heart.
  • Sequence similaritiesBelongs to the PDK/BCKDK protein kinase family.
    Contains 1 histidine kinase domain.
  • Cellular localizationMitochondrion matrix.
  • Information by UniProt

Recombinant human Mitochondrial Pyruvate dehydrogenase kinase 1 protein images

  • SDS Page analysis of ab125580
  • The specific activity of ab125580 was determined to be 1.7 nmol/min/mg.

References for Recombinant human Mitochondrial Pyruvate dehydrogenase kinase 1 protein (ab125580)

ab125580 has not yet been referenced specifically in any publications.

Product Wall

Yes, the pre-formulated kinase assay buffer is product ab189135.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"