Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Molecular weight
      83 kDa including tags
    • Amino acids
      1 to 488

Associated products

Specifications

Our Abpromise guarantee covers the use of ab125630 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    The specific activity of MLK3 was determined to be 9.5 nmol/min/mg.
  • Applications

    Western blot

    Functional Studies

    SDS-PAGE

  • Purity
    > 75 % Densitometry.
    75% by Densitometry
  • Form
    Liquid
  • Additional notes

    ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.88% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • 2610017K16Rik
    • EC 2.7.11.25
    • M3K11_HUMAN
    • Map3k11
    • MEKK11
    • MGC17114
    • Mitogen-activated protein kinase kinase kinase 11
    • Mixed lineage kinase 3
    • Mixed lineage protein kinase 3
    • Mlk-3
    • Mlk3
    • Protein tyrosine kinase PTK1
    • PTK1
    • RHOE
    • SH3 domain containing proline rich kinase
    • SPRK
    • Src-homology 3 domain-containing proline-rich kinase
    see all
  • Function
    Activates the JUN N-terminal pathway. Required for serum-stimulated cell proliferation and for mitogen and cytokine activation of MAPK14 (p38), MAPK3 (ERK) and MAPK8 (JNK1). Plays a role in mitogen-stimulated phosphorylation and activation of BRAF, but does not phosphorylate BRAF directly. Influences microtubule organization during the cell cycle.
  • Tissue specificity
    Expressed in a wide variety of normal and neoplastic tissues including fetal lung, liver, heart and kidney, and adult lung, liver, heart, kidney, placenta, skeletal muscle, pancreas and brain.
  • Sequence similarities
    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH3 domain.
  • Post-translational
    modifications
    Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation. Thr-277 is likely to be the main autophosphorylation site. Phosphorylation of Ser-555 and Ser-556 is induced by CDC42.
  • Cellular localization
    Cytoplasm > cytoskeleton > centrosome. Location is cell cycle dependent.
  • Information by UniProt

Images

  • SDS-PAGE of Human MLK3 protein.
  • The specific activity of MLK3 was determined to be 9.5 nmol /min/mg.

References

ab125630 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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