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Our Abpromise guarantee covers the use of ab124850 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
The activity was measured by its ability to cleave a fluorogenic peptide substrate Mca-KPLGL-Dpa-AR-NH2. The specific activity is > 400 pmoles/min/µg.
Recombinant Human MMP-1 protein pro form needs to be activated with p-aminophenylmercuric acetate (APMA).
1. Dilute MMP1 to 50µg/ml in the Assay Buffer: 50mM Tris, 10mM CaCl2, 150mM NaCl, 0.05% (w/v) and Brij 35, pH 7.5.
2. Activate MMP1 by adding APMA to a final concentration of 1mM.
3. Incubate at 37°C for 2 hours.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Constituents: 25% MES, 25% Brij, 25% Glycerol, 25% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ab124850 has not yet been referenced specifically in any publications.