Recombinant human MMP1 protein (ab124850)

Overview

Description

  • Nature
    Recombinant
  • Source
    HEK 293 cells
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Molecular weight
      52 kDa including tags
    • Amino acids
      1 to 469
    • Tags
      His tag C-Terminus
    • Additional sequence information
      MMP-1 Human Recombinant produced in HEK293 cells is a proform of the Human MMP1 (Met1-Asn469) and fused with a polyhistide tag at the C-terminus.

Specifications

Our Abpromise guarantee covers the use of ab124850 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    The activity was measured by its ability to cleave a fluorogenic peptide substrate Mca-KPLGL-Dpa-AR-NH2. The specific activity is > 400 pmoles/min/µg.
  • Applications

    SDS-PAGE

    Functional Studies

  • Purity
    > 95 % SDS-PAGE.
    ab124850 is purified by proprietary chromatographic techniques and 0.2µm sterile filtered.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

    Constituents: 25% MES, 25% Brij, 25% Glycerol, 25% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • 27 kDa interstitial collagenase
    • CLG
    • CLGN
    • collagenase, fibroblast
    • collagenase, interstitial
    • Fibroblast collagenase
    • Interstitial collagenase
    • Matrix metallopeptidase 1 (interstitial collagenase)
    • Matrix metalloprotease 1
    • Matrix Metalloproteinase 1
    • Matrix metalloproteinase-1
    • MMP 1
    • MMP-1
    • MMP1
    • MMP1_HUMAN
    • OTTHUMP00000045866
    see all
  • Function
    Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity.
  • Sequence similarities
    Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • Domain
    There are two distinct domains in this protein; the catalytic N-terminal, and the C-terminal which is involved in substrate specificity and in binding TIMP (tissue inhibitor of metalloproteinases).
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    modifications
    Undergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt

References

ab124850 has not yet been referenced specifically in any publications.

Customer reviews and Q&As



It is provided as an active protein.

Thank you for enquiry.

The concentration of our batch number currently available is 0.43 mg/ml.

I hope this helps and if we can assist further, please do not hesitate to contact us.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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