Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      MSPAPRPPRCLLLPLLTLGTALASLGSAQSSSFSPEAWLQQYGYLPPGDL RTHTQRSPQSLSAAIAAMQKFYGLQVTGKADADTMKAMRRPRCGVPDKFG AEIKANVRRKRYAIQGLKWQHNEITFCIQNYTPKVGEYATYEAIRKAFRV WESATPLRFREVPYAYIREGHEKQADIMIFFAEGFHGDSTPFDGEGGFLA HAYFPGPNIGGDTHFDSAEPWTVRNEDLNGNDIFLVAVHELGHALGLEHS SDPSAIMAPFYQWMD
    • Molecular weight
      31 kDa including tags
    • Amino acids
      1 to 265
    • Tags
      His tag C-Terminus

Specifications

Our Abpromise guarantee covers the use of ab168081 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Specific Activity = =60mU/mg protein after trypsin activation. One unit is defined as the amount of enzyme that hydrolyzes 1µmol Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 per min. at 37°C, pH 7.5.
  • Applications

    Functional Studies

    SDS-PAGE

  • Purity
    >90% by SDS-PAGE.

  • Form
    Liquid
  • Additional notes
    Activation: An aliquot of 5µg MMP14 proenzyme is mixed with 1µl trypsin solution in 100µl avtivation buffer and incubated for 12 min. at 25°C. Thereafter trypsin is inhibited by addition of 1µl aprotinin solution.
    Activation buffer: 50mM TRIS-HCl, pH 7.5, 150mM NaCl, 5mM CaCl2. Store at -20°C.
    Trypsin solution: 50µg TPCK-trypsin per ml activation buffer. Store in aliquots at -20°C.
    Aprotinin solution: 1mg aprotinin per ml activation buffer. Store at -20°C.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.06% Calcium chloride, 0.79% Tris HCl, 0.88% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • Matrix metallopeptidase 14 (membrane inserted)
    • Matrix metalloproteinase 14
    • Matrix metalloproteinase-14
    • Membrane type 1 matrix metalloproteinase
    • Membrane type 1 metalloprotease
    • Membrane type matrix metalloproteinase 1
    • Membrane-type matrix metalloproteinase 1
    • Membrane-type-1 matrix metalloproteinase
    • MMP 14
    • MMP X1
    • MMP-14
    • MMP-X1
    • Mmp14
    • MMP14_HUMAN
    • MMPX1
    • MT MMP 1
    • MT-MMP 1
    • MT1 MMP
    • MT1-MMP
    • MT1MMP
    • MTMMP 1
    • MTMMP1
    see all
  • Function
    Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. May be involved in actin cytoskeleton reorganization by cleaving PTK7.
  • Tissue specificity
    Expressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors.
  • Sequence similarities
    Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • Domain
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    modifications
    The precursor is cleaved by a furin endopeptidase.
  • Cellular localization
    Membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

References

ab168081 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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