Recombinant human MNK1 protein (ab125635)

Overview

Description

  • NatureRecombinant
  • SourceBaculovirus infected Sf9 cells
  • Amino Acid Sequence
    • AccessionQ9BUB5
    • SpeciesHuman
    • Molecular weight92 kDa including tags
    • Amino acids1 to 465

Specifications

Our Abpromise guarantee covers the use of ab125635 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityThe specific activity of ab125635 was determined to be 16 - 20 nmol/min/mg.
  • Applications

    Western blot

    Functional Studies

    SDS-PAGE

  • Purity> 85 % Densitometry.
    Purity was determined to be >85% by densitometry.
  • FormLiquid
  • Additional notes

    ab204868 (Modified EIF2S peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.88% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • MAP kinase interacting kinase 1
    • MAP kinase interacting serine/threonine kinase 1
    • MAP kinase signal integrating kinase 1
    • MAP kinase signal-integrating kinase 1
    • MAP kinase-interacting serine/threonine-protein kinase 1
    • MAPK signal integrating kinase 1
    • MITOGEN-ACTIVATED PROTEIN KINASE-INTERACTING SERINE/THREONINE KINASE 1
    • mknk1
    • MKNK1_HUMAN
    • MNK 1
    • Mnk1
    see all
  • FunctionMay play a role in the response to environmental stress and cytokines. Appears to regulate transcription by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Dual phosphorylation of Thr-250 and Thr-255 activates the kinase. Phosphorylation of Thr-385 activates the kinase.
  • Cellular localizationCytoplasm and Cytoplasm. Nucleus.
  • Information by UniProt

Recombinant human MNK1 protein images

  • Sample Kinase Assay showing the specific activity of ab125635 as 16 nmol/min/mg.
  • SDS-PAGE analysis of ab125635.

References for Recombinant human MNK1 protein (ab125635)

ab125635 has not yet been referenced specifically in any publications.

Product Wall

ab125635 activity was determined using substrate modified-EIF2S peptide.
ab51441 activity was determined using myelin basic protein.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"