Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Species
      Human

Specifications

Our Abpromise guarantee covers the use of ab62572 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    Western blot

  • Form
    Liquid
  • Additional notes
    ab62572 was expressed in a Baculovirus infected Sf9 cell expression system.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 50% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 8

General Info

  • Alternative names
    • MAP kinase interacting kinase 1
    • MAP kinase interacting serine/threonine kinase 1
    • MAP kinase signal integrating kinase 1
    • MAP kinase signal-integrating kinase 1
    • MAP kinase-interacting serine/threonine-protein kinase 1
    • MAPK signal integrating kinase 1
    • MITOGEN-ACTIVATED PROTEIN KINASE-INTERACTING SERINE/THREONINE KINASE 1
    • mknk1
    • MKNK1_HUMAN
    • MNK 1
    • Mnk1
    see all
  • Function
    May play a role in the response to environmental stress and cytokines. Appears to regulate transcription by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Dual phosphorylation of Thr-250 and Thr-255 activates the kinase. Phosphorylation of Thr-385 activates the kinase.
  • Cellular localization
    Cytoplasm and Cytoplasm. Nucleus.
  • Information by UniProt

Images

  • ab62572 on 10% SDS-PAGE, Coomassie staining.

    Lane 1 ab62572.

    Lane 2 Protein marker.

  • Anti-MNK1 antibody (ab60204) at 1/500 dilution + Recombinant Human MNK1 protein (ab62572) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 30 seconds

References

ab62572 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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