Recombinant human MNK2 protein (ab51441)

Overview

Description

  • NatureRecombinant
  • SourceBaculovirus infected Sf9 cells
  • Amino Acid Sequence
    • SpeciesHuman
    • TagsGST tag N-Terminus

Associated products

Specifications

Our Abpromise guarantee covers the use of ab51441 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • FormLiquid
  • Additional notes

    ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • G protein coupled receptor kinase 7
    • GPRK7
    • MAP kinase interacting serine threonine kinase
    • MAP kinase interacting serine threonine kinase 2
    • MAP kinase interacting serine threonine protein kinase 2
    • MAP kinase signal integrating kinase 2
    • MAP kinase signal-integrating kinase 2
    • MAP kinase-interacting serine/threonine-protein kinase 2
    • MAPK interacting kinase 2
    • MAPK signal integrating kinase 2
    • Mitogen activated protein kinase interacting serine threonine kinase 2
    • Mknk2
    • MKNK2_HUMAN
    • Mnk2
    • Putative map kinase interacting kinase
    see all
  • FunctionMay play a role in the response to environmental stress and cytokines. Appears to regulate transcription by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap.
  • Tissue specificityUbiquitously expressed in all tissues examined. Isoform 2 is expressed at higher levels in the ovary than is isoform 1.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Dual phosphorylation of Thr-244 and Thr-249 activates the kinase. Phosphorylation of Thr-379 activates the kinase.
  • Information by UniProt

Recombinant human MNK2 protein images

  • Kinase activity assay - specific activity 95 nmol/min/mg.
  • Recombinant human MNK2 protein (tagged). Molecular weight 74 kDa.

References for Recombinant human MNK2 protein (ab51441)

ab51441 has not yet been referenced specifically in any publications.

Product Wall

ab125635 activity was determined using substrate modified-EIF2S peptide.
ab51441 activity was determined using myelin basic protein.

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