Recombinant human MNK2 protein (ab51441)

Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Species
      Human
    • Tags
      GST tag N-Terminus

Associated products

Specifications

Our Abpromise guarantee covers the use of ab51441 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Form
    Liquid
  • Additional notes

    ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • G protein coupled receptor kinase 7
    • GPRK7
    • MAP kinase interacting serine threonine kinase
    • MAP kinase interacting serine threonine kinase 2
    • MAP kinase interacting serine threonine protein kinase 2
    • MAP kinase signal integrating kinase 2
    • MAP kinase signal-integrating kinase 2
    • MAP kinase-interacting serine/threonine-protein kinase 2
    • MAPK interacting kinase 2
    • MAPK signal integrating kinase 2
    • Mitogen activated protein kinase interacting serine threonine kinase 2
    • Mknk2
    • MKNK2_HUMAN
    • Mnk2
    • Putative map kinase interacting kinase
    see all
  • Function
    May play a role in the response to environmental stress and cytokines. Appears to regulate transcription by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap.
  • Tissue specificity
    Ubiquitously expressed in all tissues examined. Isoform 2 is expressed at higher levels in the ovary than is isoform 1.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Dual phosphorylation of Thr-244 and Thr-249 activates the kinase. Phosphorylation of Thr-379 activates the kinase.
  • Information by UniProt

Images

  • Kinase activity assay - specific activity 95 nmol/min/mg.
  • Recombinant human MNK2 protein (tagged). Molecular weight 74 kDa.

References

ab51441 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

ab125635 activity was determined using substrate modified-EIF2S peptide.
ab51441 activity was determined using myelin basic protein.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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