The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
% SDS-PAGE. ab113139 was purified using conventional chromatography.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.00 Constituents: 0.32% Tris HCl, 20% Glycerol, 0.02% DTT
Myosin regulatory light chain 12A
Myosin regulatory light chain 2
Myosin regulatory light chain 2, nonsarcomeric
Myosin regulatory light chain MRCL3
Myosin regulatory light chain MRLC3
Myosin, light polypeptide, regulatory, non sarcomeric (20kD)
FunctionMyosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion.
Sequence similaritiesContains 3 EF-hand domains.
Post-translational modificationsPhosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge.