Overview

  • Product name
    Recombinant Human MTH1 protein
  • Protein length
    Full length protein

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      MGSSHHHHHHSSGLVPRGSHMGASRLYTLVLVLQPQRVLLGMKKRGFGAG RWNGFGGKVQEGETIEDGARRELQEESGLTVDALHKVGQIVFEFVGEPEL MDVHVFCTDSIQGTPVESDEMRPCWFQLDQIPFKDMWPDDSYWFPLLLQK KKFHGYFKFQGQDTILDYTLREVDTV
    • Molecular weight
      20 kDa including tags
    • Amino acids
      1 to 156
    • Tags
      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab99390 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Mass Spectrometry

  • Mass spectrometry
    MALDI-TOF
  • Purity
    > 95 % SDS-PAGE.
    ab99390 is purified using conventional chromatography techniques.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Glycerol, 20mM Tris HCl, 2mM DTT, 100mM Sodium chloride, pH 8.0

General Info

  • Alternative names
    • 2-hydroxy-dATP diphosphatase
    • 7
    • 7 8 dihydro 8 oxoguanine triphosphatase
    • 8 oxo 7 8 dihydrodeoxyguanosine triphosphatase
    • 8 oxo 7 8 dihydroguanosine triphosphatase
    • 8 oxo dGTPase
    • 8-dihydro-8-oxoguanine triphosphatase
    • 8-oxo-dGTPase
    • 8ODP_HUMAN
    • MTH 1
    • MTH1
    • MutT human homolog 1
    • Nucleoside diphosphate linked moiety X motif 1
    • Nucleoside diphosphate linked moiety X type motif 1
    • Nucleoside diphosphate-linked moiety X motif 1
    • Nudix (nucleoside diphosphate linked moiety X) type motif 1
    • Nudix hydrolase 1
    • Nudix motif 1
    • Nudix type motif 1
    • NUDT 1
    • Nudt1
    see all
  • Function
    Antimutagenic. Acts as a sanitizing enzyme for oxidized nucleotide pools, thus suppressing cell dysfunction and death induced by oxidative stress. Hydrolyzes 8-oxo-dGTP, 8-oxo-dATP and 2-OH-dATP, thus preventing misincorporation of oxidized purine nucleoside triphosphates into DNA and subsequently preventing A:T to C:G and G:C to T:A transversions. Able to hydrolyze also the corresponding ribonucleotides, 2-OH-ATP, 8-oxo-GTP and 8-oxo-ATP. Does not play a role in U8 snoRNA decapping activity. Binds U8 snoRNA.
  • Tissue specificity
    Widely expressed with highest expression in thymus, testis, embryo and proliferating blood lymphocytes.
  • Sequence similarities
    Belongs to the Nudix hydrolase family.
    Contains 1 nudix hydrolase domain.
  • Developmental stage
    In peripheral blood lymphocytes, expressed at much higher levels in proliferating cells than in resting cells.
  • Post-translational
    modifications
    The N-terminus is blocked.
  • Cellular localization
    Cytoplasm. Mitochondrion matrix and Cytoplasm. Mitochondrion matrix. Nucleus. Mostly present in cytoplasm. Variant Met-124 has decreased efficiency in translocation to mitochondria.
  • Information by UniProt

Images

  • 15% SDS-PAGE analysis of 3µg ab99390.

References

ab99390 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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