Recombinant Human MYL9 protein (ab104014)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionP24844
    • SpeciesHuman
    • SequenceMGSSHHHHHH SSGLVPRGSH MSSKRAKAKT TKKRPQRATS NVFAMFDQSQ IQEFKEAFNM IDQNRDGFID KEDLHDMLAS LGKNPTDEYL EGMMSEAPGP INFTMFLTMF GEKLNGTDPE DVIRNAFACF DEEASGFIHE DHLRELLTTM GDRFTDEEVD EMYREAPIDK KGNFNYVEFT RILKHGAKDK DD
    • Molecular weight22 kDa
    • Amino acids1 to 172
    • TagsHis tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab104014 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Mass Spectrometry

    SDS-PAGE

    Western blot

  • Mass spectrometry
    MALDI-TOF
  • Purity> 90 % SDS-PAGE.
    Purified by using anion-exchange chromatography (DEAE sepharose resin) and gel-filtration chromatography (Sephacryl S-200) with 20mM Tris pH 7.5, 2mM EDTA.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 10% Glycerol, 20mM Tris HCl, 100mM Sodium chloride, 1mM DTT, pH 8

General Info

  • Alternative names
    • 20 kDa myosin light chain
    • Human 20kDa myosin light chain (MLC2) mRNA complete cds
    • LC20
    • MGC3505
    • MLC 2
    • MLC-2C
    • MLC2
    • MLY 9
    • MRLC1
    • MYL9
    • MYL9_HUMAN
    • Myosin light chain 9 regulatory
    • Myosin light polypeptide 9 regulatory
    • myosin regulatory light chain 1
    • Myosin regulatory light chain 2
    • Myosin regulatory light chain 2 smooth muscle isoform
    • Myosin regulatory light chain 9
    • Myosin regulatory light chain MRLC1
    • Myosin regulatory light polypeptide 9
    • Myosin RLC
    • Myosin vascular smooth muscle light chain 2
    • MYRL2
    • OTTHUMP00000030857
    • smooth muscle isoform
    see all
  • FunctionMyosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion.
  • Tissue specificitySmooth muscle tissues and in some, but not all, nonmuscle cells.
  • Sequence similaritiesContains 3 EF-hand domains.
  • Post-translational
    modifications
    Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge.
  • Information by UniProt

Recombinant Human MYL9 protein images

  • 15% SDS-PAGE analysis of ab104014 (3 µg)
  • Anti-MYL9 antibody (ab64161) at 1/2000 dilution + Recombinant Human MYL9 protein (ab104014) at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 4 minutes

References for Recombinant Human MYL9 protein (ab104014)

ab104014 has not yet been referenced specifically in any publications.

Product Wall

Application Other

Review text: This recombinant protein was successfully used as a substrate for in vitro phosphorylation at Serine 19. A phospho-specific antibody was used as a readout on a western blot.

The supplied protein was not phosphorylated at Serine 19 when received from Abcam.
Sample: Human Recombinant protein

Primary antibody (in addition to 'MYL9 protein')
Primary antibody: None used

Secondary antibody
Secondary antibody: None used

Additional data
PubMed ID: 21907820: View
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Submitted Nov 15 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"