Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Species
      Human

Associated products

Specifications

Our Abpromise guarantee covers the use of ab84800 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Specific activity: 270nmol/min/mg.
  • Applications

    Western blot

    Functional Studies

  • Form
    Liquid
  • Additional notes

    ab60014 (Mouse MYL9 full length protein) can be utilized as a substrate for assessing Kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.00
    Preservative: 1.02% Imidazole
    Constituents: 0.00174% PMSF, 0.82% Sodium phosphate, 0.00308% DTT, 25% Glycerol, 1.74% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • KMLC
    • MLCK
    • MLCK, skeletal/cardiac
    • MLCK2
    • MYLK 2
    • MYLK2
    • MYLK2_HUMAN
    • Myosin light chain kinase 2
    • Myosin light chain kinase 2 skeletal muscle
    • Myosin light chain kinase 2 skeletal/cardiac muscle
    • MYOSIN LIGHT POLYPEPTIDE KINASE
    • Myosin light polypeptide kinase, skeletal/cardiac
    • Skeletal muscle myosin light chain kinase
    • Skeletal myosin light chain kinase
    • skeletal/cardiac muscle
    • skMLCK
    see all
  • Function
    Implicated in the level of global muscle contraction and cardiac function. Phosphorylates a specific serine in the N-terminus of a myosin light chain.
  • Tissue specificity
    Heart and skeletal muscles. Increased expression in the apical tissue compared to the interventricular septal tissue.
  • Involvement in disease
    Defects in MYLK2 are a cause of familial hypertrophic cardiomyopathy (CMH) [MIM:192600]; also designated FHC or HCM. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • Cellular localization
    Cytoplasm. Colocalizes with phosphorylated myosin light chain (RLCP) at filaments of the myofibrils.
  • Information by UniProt

Images

  • Specific activity of ab84800
  • SDS page analysis of ab84800

References

ab84800 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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