Recombinant human Myosin IIIB protein (ab55716)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% Densitometry
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human Myosin IIIB protein -
Biological activity
Specific Activity: 85 nmol/min/mg
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Purity
> 90 % Densitometry.
Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Amino acids
1 to 326
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Associated products
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Substrate reagent
Specifications
Our Abpromise guarantee covers the use of ab55716 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Liquid -
Additional notes
ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- A430065P19Rik
- Myo3b
- MYO3B_HUMAN
see all -
Function
Probable actin-based motor with a protein kinase activity. -
Tissue specificity
Expressed in retina, kidney and testis. -
Sequence similarities
In the N-terminal section; belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family.
Contains 2 IQ domains.
Contains 1 myosin head-like domain.
Contains 1 protein kinase domain. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
Images
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The specific activity of Myosin IIIB (ab55716) was determined to be 78 nmol/min/mg as per activity assay protocol
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SDS PAGE analysis of ab55716
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Kinase assay using ab55716. Specific activity 85 nmol/min/mg.
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SDS-PAGE analysis of ab57716 with molecular weight markers. Approximate molecular weight 63kDa.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab55716 has not yet been referenced specifically in any publications.