Recombinant Human Myosin Light Chain 2 protein (ab79185)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Species
      Human
    • Sequence
      MGSSHHHHHH SSGLVPRGSH MAPKKAKKRA GGANSNVFSM FEQTQIQEFK EAFTIMDQNR DGFIDKNDLR DTFAALGRVN VKNEEIDEMI KEAPGPINFT VFLTMFGEKL KGADPEETIL NAFKVFDPEG KGVLKADYVR EMLTTQAERF SKEEVDQMFA AFPPDVTGNL DYKNLVHIIT HGEEKD

Specifications

Our Abpromise guarantee covers the use of ab79185 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Western blot

  • Purity
    > 95 % SDS-PAGE.
    ab79185 is purified using conventional chromatography techniques.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.0555% Calcium chloride, 0.242% Tris, 40% Glycerol

General Info

  • Alternative names
    • Cardiac myosin light chain-2
    • Cardiac ventricular myosin light chain 2
    • CMH10
    • MLC 2v
    • MLC-2
    • MLC-2v
    • MLC2
    • MLRV_HUMAN
    • MYL 2
    • MYL2
    • Myosin light chain 2 regulatory cardiac slow
    • Myosin light polypeptide 2 regulatory cardiac slow
    • Myosin regulatory light chain 2
    • Myosin regulatory light chain 2 ventricular/cardiac muscle isoform
    • Regulatory light chain of myosin
    • RLC of myosin
    • Slow cardiac myosin regulatory light chain 2
    • ventricular/cardiac muscle isoform
    see all
  • Involvement in disease
    Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic type 10 (CMH10) [MIM:608758]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
    Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic with mid-left ventricular chamber type 2 (MVC2) [MIM:608758]. MVC2 is a very rare variant of familial hypertrophic cardiomyopathy, characterized by mid-left ventricular chamber thickening.
  • Sequence similarities
    Contains 3 EF-hand domains.
  • Post-translational
    modifications
    N-terminus is methylated by METTL11A/NTM1.
  • Information by UniProt

Images

  • 15% SDS-PAGE showing ab79185 at approximately 21kDa (3µg).
  • All lanes : Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721) at 1/10000 dilution

    Lane 1 : Recombinant Human Myosin Light Chain 2 protein (ab79185) at 0.1 µg
    Lane 2 : Recombinant Human Myosin Light Chain 2 protein (ab79185) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 4 minutes

References

ab79185 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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