Recombinant human NAK/TBK1 protein (ab85276)

Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Species
      Human
    • Sequence
      MQSTSNHLWLLSDILGQGATANVFRGRHKKTGDLFAIKVFNNISFLRPVD VQMREFEVLK KLNHKNIVKLFAIEEETTTRHKVLIMEFCPCGSLYTVL EEPSNAYGLPESEFLIVLRDVV GGMNHLRENGIVHRDIKPGNIMRVIG EDGQSVYKLTDFGAARELEDDEQFVSLYGTEEYL HPDMYERAVLRKDH QKKYGATVDLWSIGVTFYHAATGSLPFRPFEGPRRNKEVMYKIITG KP SGAISGVQKAENGPIDWSGDMPVSCSLSRGLQVLLTPVLANILEADQEKC WGFDQFFA ETSDILHRMVIHVFSLQQMTAHKIYIHSYNTATIFHELVY KQTKIISSNQELIYEGRRLV LEPGRLAQHFPKTTEENPIFVVSREPLN TIGLIYEKISLPKVHPRYDLDGDASMAKAITG VVCYACRIASTLLLYQ ELMRKGIRWLIELIKDDYNETVHKKTEVVITLDFCIRNIEKTVK VYEK LMKINLEAAELGEISDIHTKLLRLSSSQGTIETSLQDIDSRLSPGGSLAD AWAHQE GTHPKDRNVEKLQVLLNCMTEIYYQFKKDKAERRLAYNEEQI HKFDKQKLYYHATKAMTH FTDECVKKYEAFLNKSEEWIRKMLHLRKQL LSLTNQCFDIEEEVSKYQEYTNELQETLPQ KMFTASSGIKHTMTPIYP SSNTLVEMTLGMKKLKEEMEGVVKELAENNHILERFGSLTMD GGLRNV DCL
    • Molecular weight
      105 kDa including tags
    • Amino acids
      1 to 729
    • Tags
      GST tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab85276 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    The Specific activity of ab85276 was determined to be 305 nmol/min/mg.
  • Applications

    Western blot

    Functional Studies

  • Form
    Liquid
  • Additional notes

    ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • EC 2.7.11.1
    • FLJ11330
    • FTDALS4
    • NAK
    • NF kappa B activating kinase
    • NF kB activating kinase
    • NF-kappa-B-activating kinase
    • Serine/threonine protein kinase TBK 1
    • Serine/threonine protein kinase TBK1
    • Serine/threonine-protein kinase TBK1
    • T2K
    • TANK binding kinase 1
    • TANK-binding kinase 1
    • TBK 1
    • Tbk1
    • TBK1_HUMAN
    see all
  • Function
    Serine/threonine protein involved in the signaling cascade converging to the activation of the transcription factor NF-kappa-B. May function as an IKK kinase, playing an essential role in the transcription of a subset of TNF-alpha-induced genes. Also mediates production of RANTES/CCL5 and interferon-beta/IFNB1. Has a pivotal role in the innate immune response. Phosphorylates Borna disease virus (BDV) P protein. Phosphorylates and activates IRF3 and IRF7 and allows their nuclear localization. This leads to production of alpha/beta interferons and the development of a cellular antiviral state. It also seems to be a central factor in the induction of the antiviral interferon response. Inhibition of its interaction with IRF3, due to HCV NS3 binding or BDV P protein seems to be one mechanism of inhibition of the innate immune responses of hepatitis C virus (HCV) infection or Borna disease virus infection respectively.
  • Tissue specificity
    Ubiquitous with higher expression in testis.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
    Contains 1 protein kinase domain.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt

Images

  • The Specific activity of ab85276 was determined to be 305 nmol/min/mg.
  • SDS-PAGE showing ab85276 at approximately 105kDa.

  • Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 1 minute

References

ab85276 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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