The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Endotoxin level< 1.000 Eu/µg
% SDS-PAGE. No detergents such as urea, Triton or Tween were used to purify this protein. Purified by using anion exchange chromatography (DEAE sepharose resin) and gel filtration chromatography (Sephacryl S 200) with 20mM Tris pH 7.5, 2mM EDTA.
This protein is released in large amounts from platelets following their activation. It stimulates various processes including mitogenesis, synthesis of extracellular matrix, glucose metabolism and synthesis of plasminogen activator.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.50 Constituents: 0.32% Tris HCl, 0.02% DTT, 10% Glycerol
C-X-C motif chemokine 7
Chemokine (C X C motif) ligand 7
Connective tissue activating peptide III
CXC chemokine ligand 7
LA PF 4
Leukocyte derived growth factor
Leukocyte-derived growth factor
Low-affinity platelet factor IV
Macrophage-derived growth factor
Neutrophil activating peptide 2
Neutrophil-activating peptide 2(1-63)
Platelet basic protein
Pro platelet basic protein
Pro platelet basic protein (chemokine (C-X-C motif) ligand 7)
Small inducible cytokine subfamily B member 7
Small-inducible cytokine B7
FunctionLA-PF4 stimulates DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by human synovial cells. NAP-2 is a ligand for CXCR1 and CXCR2, and NAP-2, NAP-2(73), NAP-2(74), NAP-2(1-66), and most potent NAP-2(1-63) are chemoattractants and activators for neutrophils. TC-1 and TC-2 are antibacterial proteins, in vitro released from activated platelet alpha-granules. CTAP-III(1-81) is more potent than CTAP-III desensitize chemokine-induced neutrophil activation.
Sequence similaritiesBelongs to the intercrine alpha (chemokine CxC) family.
Post-translational modificationsProteolytic removal of residues 1-9 produces the active peptide connective tissue-activating peptide III (CTAP-III) (low-affinity platelet factor IV (LA-PF4)). Proteolytic removal of residues 1-13 produces the active peptide beta-thromboglobulin, which is released from platelets along with platelet factor 4 and platelet-derived growth factor. NAP-2(1-66) is produced by proteolytical processing, probably after secretion by leukocytes other than neutrophils. NAP-2(73) and NAP-2(74) seem not be produced by proteolytical processing of secreted precursors but are released in an active form from platelets.
SDS-PAGE analysis of ab109834: (1) MW marker, (2) ab109834 at 3µg Gel concentration: 15%
References for Recombinant Human NAP2 protein (ab109834)
This product has been referenced in:
Majumdar S et al. Characterization of the human beta-thromboglobulin gene. Comparison with the gene for platelet factor 4. J Biol Chem266:5785-9 (1991).
Read more (PubMed: 1826003) »
Holt JC et al. Characterization of human platelet basic protein, a precursor form of low-affinity platelet factor 4 and beta-thromboglobulin. Biochemistry25:1988-96 (1986).
Read more (PubMed: 2423119) »