Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionP02775
    • SpeciesHuman
    • SequenceMSSTKGQTKR NLAKGKEESL DSDLYAELRC MCIKTTSGIH PKNIQSLEVI GKGTHCNQVE VIATLKDGRK ICLDPDAPRI KKIVQKKLAG DESAD
    • Molecular weight10 kDa
    • Amino acids35 to 128

Specifications

Our Abpromise guarantee covers the use of ab109834 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Mass Spectrometry

  • Endotoxin level< 1.000 Eu/µg
  • Mass spectrometry
    MALDI-TOF
  • Purity> 90 % SDS-PAGE.
    No detergents such as urea, Triton or Tween were used to purify this protein. Purified by using anion exchange chromatography (DEAE sepharose resin) and gel filtration chromatography (Sephacryl S 200) with 20mM Tris pH 7.5, 2mM EDTA.
  • FormLiquid
  • Additional notes


    This protein is released in large amounts from platelets following their activation. It stimulates various processes including mitogenesis, synthesis of extracellular matrix, glucose metabolism and synthesis of plasminogen activator.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 7.50
    Constituents: 0.32% Tris HCl, 0.02% DTT, 10% Glycerol

General Info

  • Alternative names
    • B TG1
    • Beta TG
    • Beta thromboglobulin
    • Beta-TG
    • C-X-C motif chemokine 7
    • Chemokine (C X C motif) ligand 7
    • Connective tissue activating peptide III
    • CTAP 3
    • CTAP III
    • CTAP-III
    • CTAP-III(1-81)
    • CTAP3
    • CTAPIII
    • CXC chemokine ligand 7
    • CXCL 7
    • CXCL7
    • CXCL7_HUMAN
    • LA PF 4
    • LA-PF4
    • LDGF
    • Leukocyte derived growth factor
    • Leukocyte-derived growth factor
    • Low-affinity platelet factor IV
    • Macrophage-derived growth factor
    • MDGF
    • NAP 2
    • NAP-2
    • NAP-2(1-63)
    • NAP-2(1-66)
    • NAP-2(73)
    • NAP-2(74)
    • Neutrophil activating peptide 2
    • Neutrophil-activating peptide 2(1-63)
    • PBP
    • Platelet basic protein
    • PPBP
    • Pro platelet basic protein
    • Pro platelet basic protein (chemokine (C-X-C motif) ligand 7)
    • SCYB7
    • Small inducible cytokine subfamily B member 7
    • Small-inducible cytokine B7
    • TC1
    • TC2
    • TGB
    • TGB1
    • THBGB
    • THBGB1
    • Thrombocidin 1
    • Thrombocidin 2
    • Thromboglobulin, beta-1
    see all
  • FunctionLA-PF4 stimulates DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by human synovial cells. NAP-2 is a ligand for CXCR1 and CXCR2, and NAP-2, NAP-2(73), NAP-2(74), NAP-2(1-66), and most potent NAP-2(1-63) are chemoattractants and activators for neutrophils. TC-1 and TC-2 are antibacterial proteins, in vitro released from activated platelet alpha-granules. CTAP-III(1-81) is more potent than CTAP-III desensitize chemokine-induced neutrophil activation.
  • Sequence similaritiesBelongs to the intercrine alpha (chemokine CxC) family.
  • Post-translational
    modifications
    Proteolytic removal of residues 1-9 produces the active peptide connective tissue-activating peptide III (CTAP-III) (low-affinity platelet factor IV (LA-PF4)).
    Proteolytic removal of residues 1-13 produces the active peptide beta-thromboglobulin, which is released from platelets along with platelet factor 4 and platelet-derived growth factor.
    NAP-2(1-66) is produced by proteolytical processing, probably after secretion by leukocytes other than neutrophils.
    NAP-2(73) and NAP-2(74) seem not be produced by proteolytical processing of secreted precursors but are released in an active form from platelets.
  • Cellular localizationSecreted.
  • Information by UniProt

Recombinant Human NAP2 protein images

  • SDS-PAGE analysis of ab109834: (1) MW marker, (2) ab109834 at 3µg
    Gel concentration: 15%

References for Recombinant Human NAP2 protein (ab109834)

This product has been referenced in:
  • Majumdar S  et al. Characterization of the human beta-thromboglobulin gene. Comparison with the gene for platelet factor 4. J Biol Chem 266:5785-9 (1991). Read more (PubMed: 1826003) »
  • Holt JC  et al. Characterization of human platelet basic protein, a precursor form of low-affinity platelet factor 4 and beta-thromboglobulin. Biochemistry 25:1988-96 (1986). Read more (PubMed: 2423119) »

See all 2 Publications for this product

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