Recombinant Human NAP2 protein (ab9556)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesHuman
    • SequenceRecombinant Human NAP-2 is a 7.6 kDa protein containing 70 amino acid residues: AELRCMCIKT TSGIHPKNIQ SLEVIGKGTH CNQVEVIATL KD GRKICLDP DAPRIKKIVQKKLAGDESAD

Associated products

Specifications

Our Abpromise guarantee covers the use of ab9556 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Endotoxin level< 0.100 Eu/µg
  • FormLyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    n/a

General Info

  • Alternative names
    • B TG1
    • Beta TG
    • Beta thromboglobulin
    • Beta-TG
    • C-X-C motif chemokine 7
    • Chemokine (C X C motif) ligand 7
    • Connective tissue activating peptide III
    • CTAP 3
    • CTAP III
    • CTAP-III
    • CTAP-III(1-81)
    • CTAP3
    • CTAPIII
    • CXC chemokine ligand 7
    • CXCL 7
    • CXCL7
    • CXCL7_HUMAN
    • LA PF 4
    • LA-PF4
    • LDGF
    • Leukocyte derived growth factor
    • Leukocyte-derived growth factor
    • Low-affinity platelet factor IV
    • Macrophage-derived growth factor
    • MDGF
    • NAP 2
    • NAP-2
    • NAP-2(1-63)
    • NAP-2(1-66)
    • NAP-2(73)
    • NAP-2(74)
    • Neutrophil activating peptide 2
    • Neutrophil-activating peptide 2(1-63)
    • PBP
    • Platelet basic protein
    • PPBP
    • Pro platelet basic protein
    • Pro platelet basic protein (chemokine (C-X-C motif) ligand 7)
    • SCYB7
    • Small inducible cytokine subfamily B member 7
    • Small-inducible cytokine B7
    • TC1
    • TC2
    • TGB
    • TGB1
    • THBGB
    • THBGB1
    • Thrombocidin 1
    • Thrombocidin 2
    • Thromboglobulin, beta-1
    see all
  • FunctionLA-PF4 stimulates DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by human synovial cells. NAP-2 is a ligand for CXCR1 and CXCR2, and NAP-2, NAP-2(73), NAP-2(74), NAP-2(1-66), and most potent NAP-2(1-63) are chemoattractants and activators for neutrophils. TC-1 and TC-2 are antibacterial proteins, in vitro released from activated platelet alpha-granules. CTAP-III(1-81) is more potent than CTAP-III desensitize chemokine-induced neutrophil activation.
  • Sequence similaritiesBelongs to the intercrine alpha (chemokine CxC) family.
  • Post-translational
    modifications
    Proteolytic removal of residues 1-9 produces the active peptide connective tissue-activating peptide III (CTAP-III) (low-affinity platelet factor IV (LA-PF4)).
    Proteolytic removal of residues 1-13 produces the active peptide beta-thromboglobulin, which is released from platelets along with platelet factor 4 and platelet-derived growth factor.
    NAP-2(1-66) is produced by proteolytical processing, probably after secretion by leukocytes other than neutrophils.
    NAP-2(73) and NAP-2(74) seem not be produced by proteolytical processing of secreted precursors but are released in an active form from platelets.
  • Cellular localizationSecreted.
  • Information by UniProt

Recombinant Human NAP2 protein images

  • Recombinant Human NAP2 protein (ab9556) at 1/1000 dilution + Human CXCL7 active recombinant protein, 10 ng

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Observed band size : 11 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 seconds

    Blocking and Diluting buffer - 5% NFDM/TBST

References for Recombinant Human NAP2 protein (ab9556)

ab9556 has not yet been referenced specifically in any publications.

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For ab9554, Rabbit Anti Human NAP-2, this has been tested in Neutralisation as follows:
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human NAP-2 (100.0 ng/ml), a c...

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