Overview

Description

  • NatureRecombinant
  • SourceBaculovirus infected Sf9 cells
  • Amino Acid Sequence
    • SpeciesHuman

Specifications

Our Abpromise guarantee covers the use of ab85711 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: 150mM Imidazole
    Constituents: 25% Glycerol, 50mM Sodium phosphate, 300mM Sodium chloride, 2mM DTT, 0.1mM PMSF, pH 7.0

General Info

  • Alternative names
    • Cytoplasmic protein NCK1
    • Melanoma Nck protein
    • MGC12668
    • NCK
    • NCK 1
    • NCK adaptor protein 1
    • NCK alpha
    • NCK tyrosine kinase
    • Nck-1
    • NCK1
    • NCK1_HUMAN
    • NCKalpha
    • Non catalytic region of tyrosine kinase
    • SH2/SH3 adaptor protein NCK alpha
    • SH2/SH3 adaptor protein NCK-alpha
    see all
  • FunctionAdapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2.
  • Sequence similaritiesContains 1 SH2 domain.
    Contains 3 SH3 domains.
  • Post-translational
    modifications
    Phosphorylated on Ser and Tyr residues.
  • Cellular localizationCytoplasm. Endoplasmic reticulum. Nucleus. Mostly cytoplasmic, but shuttles between the cytoplasm and the nucleus. Import into the nucleus requires the interaction with SOCS7. Predominantly nuclear following genotoxic stresses, such as UV irradiation, hydroxyurea or mitomycin C treatments.
  • Information by UniProt

Recombinant Human Nck protein images

  • SDS-PAGE showing ab85711 at approximately 48kDa.

References for Recombinant Human Nck protein (ab85711)

ab85711 has not yet been referenced specifically in any publications.

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