Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesHuman
    • SequenceMSREMQDVDL AEVKPLVEKG ETITGLLQEF DVQEQDIETL HGSVHVTLCG TPKGNRPVIL TYHDIGMNHK TCYNPLFNYE DMQEITQHFA VCHVDAPGQQ DGAASFPAGY MYPSMDQLAE MLPGVLQQFG LKSIIGMGTG AGAYILTRFA LNNPEMVEGL VLINVNPCAE GWMDWAASKI SGWTQALPDM VVSHLFGKEE MQSNVEVVHT YRQHIVNDMN PGNLHLFINA YNSRRDLEIE RPMPGTHTVT LQCPALLVVG DSSPAVDAVV ECNSKLDPTK TTLLKMADCG GLPQISQPAK LAEAFKYFVQ GMGYMPSASM TRLMRSRTAS GSSVTSLDGT RSRSHTSEGT RSRSHTSEGT RSRSHTSEGA HLDITPNSGA AGNSAGPKSM EVSCLEHHHH HH

Specifications

Our Abpromise guarantee covers the use of ab87685 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityThe ED50 for this effect is 0.5 - 1.5 ng/ml. Measured in a cell proliferation assay using MCF7 cell.

    Activity Assay

    1. Cell line: MCF7 (Human breast adenocarcinoma cell)
    2. Maintenance Condition: RPMI 1640 containing 10% FBS
    3. Assay medium: serum free RPMI 1640
    4. Cell density: 2 x 104 cells/well (96 well plate, final volume 100ul)
    5. Incubation time : 40 hrs (after sample treatment)
    6. Concentration range: 0.19ng/ml - 50ng/ml
    7. Detection method : BrdU assay
  • Applications

    SDS-PAGE

  • Mass spectrometry
    MALDI-TOF
  • Purity> 95 % SDS-PAGE.
    ab87685 is purified using conventional chromatography.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Glycerol, 20mM Tris HCl, 0.1mM PMSF, pH 8.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • 42 kDa
    • Anti GC4
    • cap43
    • cmt4d
    • Differentiation related gene1 protein
    • Differentiation-related gene 1 protein
    • Drg 1
    • DRG-1
    • drg1
    • gc4
    • GC4 antibody
    • hmsnl
    • Human mRNA for RTP complete cds
    • N myc downstream regulated gene 1
    • N myc downstream regulated gene 1 protein
    • N-myc downstream-regulated gene 1 protein
    • Ndr 1
    • ndr1
    • NDRG 1
    • Ndrg1
    • NDRG1 protein
    • NDRG1_HUMAN
    • Nickel specific induction protein
    • Nickel specific induction protein Cap43
    • Nickel-specific induction protein Cap43
    • nmsl
    • Nmyc downstream regulated
    • Nmyc downstream regulated gene1
    • Nmyc downstream regulated gene1 protein
    • Protein NDRG1
    • Protein regulated by oxygen 1
    • Protein regulated by oxygen1
    • Proxy1
    • Reduced in tumor
    • Reducin
    • Reducing agents and tunicamycin responsive protein
    • Reducing agents and tunicamycin-responsive protein
    • Rit42
    • RTP
    • targ1
    • TDD5
    • tdds
    • Tunicamycin responsive protein
    see all
  • FunctionMay have a growth inhibitory role.
  • Tissue specificityUbiquitous; expressed most prominently in placental membranes and prostate, kidney, small intestine, and ovary tissues. Reduced expression in adenocarcinomas compared to normal tissues. In colon, prostate and placental membranes, the cells that border the lumen show the highest expression.
  • Involvement in diseaseDefects in NDRG1 are the cause of Charcot-Marie-Tooth disease type 4D (CMT4D) [MIM:601455]; also known as hereditary motor and sensory neuropathy Lom type (HMSNL). CMT4D is a recessive form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy and primary peripheral axonal neuropathy. Demyelinating CMT neuropathies are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet. By convention, autosomal recessive forms of demyelinating Charcot-Marie-Tooth disease are designated CMT4.
  • Sequence similaritiesBelongs to the NDRG family.
  • Cellular localizationCytoplasm. Nucleus. Cell membrane. Whereas in prostate epithelium and placental chorion it is located in both the cytoplasm and the nucleus, nuclear staining is not observed in colon epithelium cells. Instead its localization changes from the cytoplasm to the plasma membrane during differentiation of colon carcinoma cell lines in vitro.
  • Information by UniProt

Recombinant human NDRG1 protein images

  • SDS-PAGE analysis of ab87685.

References for Recombinant human NDRG1 protein (ab87685)

ab87685 has not yet been referenced specifically in any publications.

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