Overview

  • Product name
    Recombinant Human NEIL2 protein
  • Protein length
    Full length protein

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      MGSSHHHHHHSSGLVPRGSHMGSHMPEGPLVRKFHHLVSPFVGQQVVKTG GSSKKLQPASLQSLWLQDTQVHGKKLFLRFDLDEEMGPPGSSPTPEPPQK EVQKEGAADPKQVGEPSGQKTLDGSSRSAELVPQGEDDSEYLERDAPAGD AGRWLRVSFGLFGSVWVNDFSRAKKANKRGDWRDPSPRLVLHFGGGGFLA FYNCQLSWSSSPVVTPTCDILSEKFHRGQALEALGQAQPVCYTLLDQRYF SGLGNIIKNEALYRAGIHPLSLGSVLSASRREVLVDHVVEFSTAWLQGKF QGRPQHTQVYQKEQCPAGHQVMKEAFGPEDGLQRLTWWCPQCQPQLSEEP EQCQFS
    • Molecular weight
      39 kDa including tags
    • Amino acids
      1 to 332
    • Tags
      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab124545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Mass Spectrometry

  • Mass spectrometry
    MALDI-TOF
  • Purity
    > 85 % SDS-PAGE.
    ab124545 was purified using conventional chromatography techniques.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.02% DTT, 0.32% Tris HCl, 10% Glycerol, 0.58% Sodium chloride

General Info

  • Alternative names
    • DNA glycosylase/AP lyase Neil2
    • DNA-(apurinic or apyrimidinic site) lyase Neil2
    • Endonuclease 8-like 2
    • Endonuclease VIII-like 2
    • NEH2
    • Nei endonuclease VIII like 2
    • Nei endonuclease VIII like 2 (E. coli)
    • Nei homolog 2
    • Nei-like 2
    • Nei-like protein 2
    • NEI2
    • NEIL2
    • NEIL2_HUMAN
    see all
  • Function
    Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Has DNA glycosylase activity towards 5-hydroxyuracil and other oxidized derivatives of cytosine with a preference for mismatched double stranded DNA (DNA bubbles). Has low or no DNA glycosylase activity towards thymine glycol, 2-hydroxyadenine, hypoxanthine and 8-oxoguanine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates.
  • Tissue specificity
    Detected in testis, skeletal muscle, heart, brain, placenta, lung, pancreas, kidney and liver.
  • Sequence similarities
    Belongs to the FPG family.
    Contains 1 FPG-type zinc finger.
  • Domain
    The zinc-finger domain is important for DNA binding.
  • Cellular localization
    Nucleus.
  • Information by UniProt

Recombinant Human NEIL2 protein images

  • 15% SDS-PAGE analysis of 3 µg ab124545.

References for Recombinant Human NEIL2 protein (ab124545)

ab124545 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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