The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological activityActivity: 70 U/mg. One unit is defined as the amount of enzyme that will phosphorylate 1 nmol NEK6 substrate (FLAKSFGSPNRAYKK) per minute at pH 7.4 and 30C. Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 uM Naorthovanadate, 0.1 mM ATP, 4 uM NEK6 substrate, and 0.4 mg/ml NEK6.
% SDS-PAGE. >90% by SDS-PAGE
Additional notesExpressed in a Baculovirus infected Sf9 cell expression system.
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Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Highly similar to cell cycle protein kinase CDC5/MSD2
Never in mitosis A-related kinase 6
NIMA (Never In Mitosis Gene A) Related Kinase 6
NimA related protein kinase 6
NimA-related protein kinase 6
Protein kinase SID6 1512
Protein kinase SID6-1512
Putative serine threonine protein kinase
Serine/threonine protein kinase Nek6
Serine/threonine-protein kinase Nek6
FunctionActivated during M phase. Required for chromosome segregation at metaphase-anaphase transition and therefore for mitotic progression. Inhibition of activity results in apoptosis. Phosphorylates KIF11 to promote mitotic spindle formation.
Tissue specificityUbiquitous, with highest expression in heart and skeletal muscle.
Sequence similaritiesBelongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily. Contains 1 protein kinase domain.