Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Species
      Human

Associated products

Specifications

Our Abpromise guarantee covers the use of ab60698 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

  • Purity
    > 90 % SDS-PAGE.
    Purity: >90% as determined by densitometry.
  • Form
    Liquid
  • Additional notes

    ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • Highly similar to cell cycle protein kinase CDC5/MSD2
    • NEK6
    • NEK6_HUMAN
    • Never in mitosis A-related kinase 6
    • NIMA (Never In Mitosis Gene A) Related Kinase 6
    • NimA related protein kinase 6
    • NimA-related protein kinase 6
    • Protein kinase SID6 1512
    • Protein kinase SID6-1512
    • Putative serine threonine protein kinase
    • Serine/threonine protein kinase Nek6
    • Serine/threonine-protein kinase Nek6
    • SID6 1512
    • SID61512
    see all
  • Function
    Activated during M phase. Required for chromosome segregation at metaphase-anaphase transition and therefore for mitotic progression. Inhibition of activity results in apoptosis. Phosphorylates KIF11 to promote mitotic spindle formation.
  • Tissue specificity
    Ubiquitous, with highest expression in heart and skeletal muscle.
  • Sequence similarities
    Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt

Images

  • Sample Kinase Activity Plot.
  • ab60698 on SDS-PAGE, MW ~63kDa.

References

ab60698 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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