Overview

Description

  • NatureRecombinant
  • SourceBaculovirus infected Sf9 cells
  • Amino Acid Sequence
    • AccessionNM_033116
    • SpeciesHuman
    • Molecular weight115 kDa including tags
    • Amino acids1 to 979
    • TagsGST tag N-Terminus
    • Additional sequence information1-979; deleted fragment of 346-731

Specifications

Our Abpromise guarantee covers the use of ab125614 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityThe specific activity of ab125614 was determined to be 120 nmol/mg/min.
  • Applications

    Western blot

    Functional Studies

    SDS-PAGE

  • Purity> 75 % SDS-PAGE.
    Purity determined to be >75% by densitometry.
  • FormLiquid
  • Additional notes

    ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.004% EGTA, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.88% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • KIAA1995
    • APUG
    • DKFZp434D0935
    • LCCS10
    • MGC138306
    • MGC16714
    • NC
    • NEK 9
    • Nek8
    • nek9
    • NEK9_HUMAN
    • NERCC
    • Nercc 1 kinase
    • NERCC1
    • Nercc1 kinase
    • Never in mitosis A related kinase 9
    • Never in mitosis A-related kinase 9
    • NIMA (never in mitosis gene a) related kinase 9
    • NIMA related kinase 8
    • NIMA related kinase 9
    • NIMA related kinase Nek8
    • NimA related protein kinase 9
    • NIMA-related kinase 8
    • NimA-related protein kinase 9
    • Serine/threonine protein kinase Nek9
    • Serine/threonine-protein kinase Nek9
    see all
  • FunctionPleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation. Phosphorylates different histones, myelin basic protein, beta-casein, and BICD2. Phosphorylates histone H3 on serine and threonine residues and beta-casein on serine residues. Important for G1/S transition and S phase progression.
  • Tissue specificityMost abundant in heart, liver, kidney and testis. Also expressed in smooth muscle cells and fibroblasts.
  • Sequence similaritiesBelongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
    Contains 1 protein kinase domain.
    Contains 6 RCC1 repeats.
  • Developmental stageExpression varied mildly across the cell cycle, with highest expression observed in G1 and stationary-phase cells.
  • DomainDimerizes through its coiled-coil domain.
  • Post-translational
    modifications
    Autophosphorylated on serine and threonine residues. When complexed with FACT, exhibits markedly elevated phosphorylation on Thr-210. During mitosis, not phosphorylated on Thr-210. Phosphorylated by CDK1 in vitro.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt

Recombinant human NEK9 protein images

  • Sample Kinase Assay showing the specific activity of ab125614 as 120 nmol/min/mg.
  • SDS-PAGE analysis of ab125614.

References for Recombinant human NEK9 protein (ab125614)

ab125614 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"