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Source Escherichia coli
Amino Acid Sequence
Sequence MGSSHHHHHHSSGLVPRGSHMLVIPPGLSEEEEALQKKFNKLKKKKKALL ALKKQSSSSTTSQGGVKRSLSEQPVMDTATATEQAKQLVKSGAISAIKAE TKNSGFKRSRTLEGKLKDPEKGPVPTFQPFQRSISADDDLQESSRRPQRK SLYESFVSSSDRLRELGPDGEEAEGPGAGDGPPRSFDWGYEERSGAHSSA SPPRSRSRDRSHERNRDRDRDRERDRDRDRDRDRERDRDRDRDRDRDRER DRDRERDRDRDREGPFRRSDSFPERRAPRKGNTLYVYGEDMTPTLLRGAF SPFGNIIDLSMDPPRNCAFVTYEKMESADQAVAELNGTQVESVQLKVNIA RKQPMLDAATGKSVWGSLAVQNSPKGCHRDKRTQIVYSDDVYKENLVDGF
Molecular weight 45 kDa including tags
Amino acids 1 to 380
Tags His tag N-Terminus Specifications
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Purity > 85
% SDS-PAGE. ab104637 is purified using conventional chromatography techniques.
Concentration information loading... Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Constituents: 10% Glycerol, 0.1M Sodium chloride, 20mM Tris HCl, 2mM DTT, pH 8.0
Major histocompatibility complex gene RD
Negative elongation factor E
Negative elongation factor polypeptide E
Function Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex.
Tissue specificity Widely expressed. Expressed in heart, brain, lung, placenta, liver, skeletal muscle, kidney and pancreas.
Sequence similarities Belongs to the RRM NELF-E family. Contains 1 RRM (RNA recognition motif) domain.
Domain The RRM domain interacts with RNA, and is essential for NELF complex function. It is however not required for the NELF complex formation.
Post-translational modifications Sumoylated.
Cellular localization Nucleus.
Information by UniProt
Recombinant Human NELFe protein images
References for Recombinant Human NELFe protein (ab104637)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"