Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionP18615
    • SpeciesHuman
    • SequenceMGSSHHHHHHSSGLVPRGSHMLVIPPGLSEEEEALQKKFNKLKKKKKALL ALKKQSSSSTTSQGGVKRSLSEQPVMDTATATEQAKQLVKSGAISAIKAE TKNSGFKRSRTLEGKLKDPEKGPVPTFQPFQRSISADDDLQESSRRPQRK SLYESFVSSSDRLRELGPDGEEAEGPGAGDGPPRSFDWGYEERSGAHSSA SPPRSRSRDRSHERNRDRDRDRERDRDRDRDRDRERDRDRDRDRDRDRER DRDRERDRDRDREGPFRRSDSFPERRAPRKGNTLYVYGEDMTPTLLRGAF SPFGNIIDLSMDPPRNCAFVTYEKMESADQAVAELNGTQVESVQLKVNIA RKQPMLDAATGKSVWGSLAVQNSPKGCHRDKRTQIVYSDDVYKENLVDGF
    • Molecular weight45 kDa including tags
    • Amino acids1 to 380
    • TagsHis tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab104637 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Mass Spectrometry

  • Mass spectrometry
    MALDI-TOF
  • Purity> 85 % SDS-PAGE.
    ab104637 is purified using conventional chromatography techniques.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Glycerol, 0.1M Sodium chloride, 20mM Tris HCl, 2mM DTT, pH 8.0

General Info

  • Alternative names
    • Major histocompatibility complex gene RD
    • Negative elongation factor E
    • Negative elongation factor polypeptide E
    • NELF e
    • NELF-E
    • Nelfe
    • NELFE_HUMAN
    • Nuclear protein
    • RD
    • RD protein
    • RD RNA binding protein
    • RDP
    • RNA-binding protein RD
    see all
  • FunctionEssential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex.
  • Tissue specificityWidely expressed. Expressed in heart, brain, lung, placenta, liver, skeletal muscle, kidney and pancreas.
  • Sequence similaritiesBelongs to the RRM NELF-E family.
    Contains 1 RRM (RNA recognition motif) domain.
  • DomainThe RRM domain interacts with RNA, and is essential for NELF complex function. It is however not required for the NELF complex formation.
  • Post-translational
    modifications
    Sumoylated.
  • Cellular localizationNucleus.
  • Information by UniProt

Recombinant Human NELFe protein images

  • 15% SDS-PAGE analysis of 3µg ab104637.
    Note: Real molecular weight on SDS-PAGE is shifted up compared to predicted molecular weight.

References for Recombinant Human NELFe protein (ab104637)

ab104637 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"