Recombinant Human Neuroglobin protein (ab63278)

Overview

  • Product nameRecombinant Human Neuroglobin protein
  • Protein lengthFull length protein

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesHuman
    • SequenceM-ERPEPELIR QSWRAVSRSP LEHGTVLFAR LFALEPDLLP LFQYNCRQFS SPEDCLSSPE FLDHIRKVML VIDAAVTNVE DLSSLEEYLA SLGRKHRAVG VKLSSFSTVG ESLLYMLEKC LGPAFTPATR AAWSQLYGAV VQAMSRGWDG E

Associated products

Specifications

Our Abpromise guarantee covers the use of ab63278 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    ELISA

    SDS-PAGE

  • Purity> 95 % SDS-PAGE.
    Please filter the product by an appropriate sterile filter before using it in the cell culture.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 0.1M Sodium chloride, 0.05M PBS, pH 7.2

General Info

  • Alternative names
    • Neuroglobin
    • NGB
    • NGB_HUMAN
  • FunctionInvolved in oxygen transport in the brain. Hexacoordinate globin, displaying competitive binding of oxygen or the distal His residue to the iron atom. Not capable of penetrating cell membranes. The deoxygenated form exhibits nitrite reductase activity inhibiting cellular respiration via NO-binding to cytochrome c oxidase. Involved in neuroprotection during oxidative stress. May exert its anti-apoptotic activity by acting to reset the trigger level of mitochondrial cytochrome c release necessary to commit the cells to apoptosis.
  • Tissue specificityPredominantly expressed in brain, the strongest expression is seen in the frontal lobe, the subthalamic nucleus and the thalamus.
  • Sequence similaritiesBelongs to the globin family.
  • Post-translational
    modifications
    A redox disulfide bond regulates the heme pocket coordination and the rate of nitrite reduction to NO.
    Phosphorylated in vitro by ERK1, ERK2 and PKA, and in vivo during hypoxia. Phosphorylation increases nitrite reductase activity.
  • Cellular localizationPerikaryon. Cytoplasm. Mitochondrion.
  • Information by UniProt

Recombinant Human Neuroglobin protein images

  • LANE 1. M.W. marker - 14, 21, 31, 45, 66, 97 kDa. LANE 2. reduced and heated sample, 5µg/lane. LANE 3. non-reduced and non-heated sample, 5µg/lane.
  • Anti-Neuroglobin antibody [13C8] (ab37258) at 1 µg/ml + Recombinant Human Neuroglobin protein (ab63278) at 0.1 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 4 minutes

References for Recombinant Human Neuroglobin protein (ab63278)

ab63278 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"